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1tex

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[[Image:1tex.jpg|left|200px]]<br /><applet load="1tex" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tex, resolution 2.60&Aring;" />
 
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'''Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose'''<br />
 
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==Overview==
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==Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose==
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Sulfolipid-1 (SL-1) is an abundant sulfated glycolipid and potential virulence factor found in Mycobacterium tuberculosis. SL-1 consists of a trehalose-2-sulfate (T2S) disaccharide elaborated with four lipids. We identified and characterized a conserved mycobacterial sulfotransferase, Stf0, which generates the T2S moiety of SL-1. Biochemical studies demonstrated that the enzyme requires unmodified trehalose as substrate and is sensitive to small structural perturbations of the disaccharide. Disruption of stf0 in Mycobacterium smegmatis and M. tuberculosis resulted in the loss of T2S and SL-1 formation, respectively. The structure of Stf0 at a resolution of 2.6 A reveals the molecular basis of trehalose recognition and a unique dimer configuration that encloses the substrate into a bipartite active site. These data provide strong evidence that Stf0 carries out the first committed step in the biosynthesis of SL-1 and establish a system for probing the role of SL-1 in M. tuberculosis infection.
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<StructureSection load='1tex' size='340' side='right'caption='[[1tex]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tex]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TEX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900006:trehalose'>PRD_900006</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tex OCA], [https://pdbe.org/1tex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tex RCSB], [https://www.ebi.ac.uk/pdbsum/1tex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tex ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P84151_MYCSM P84151_MYCSM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1tex_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tex ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TEX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with <scene name='pdbligand=TRE:'>TRE</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEX OCA].
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*[[Sulfotransferase 3D structures|Sulfotransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis., Mougous JD, Petzold CJ, Senaratne RH, Lee DH, Akey DL, Lin FL, Munchel SE, Pratt MR, Riley LW, Leary JA, Berger JM, Bertozzi CR, Nat Struct Mol Biol. 2004 Aug;11(8):721-9. Epub 2004 Jul 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15258569 15258569]
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[[Category: Large Structures]]
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[[Category: Mycobacterium smegmatis]]
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[[Category: Mycolicibacterium smegmatis]]
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[[Category: Single protein]]
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[[Category: Akey DL]]
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[[Category: Akey, D L.]]
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[[Category: Berger JM]]
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[[Category: Berger, J M.]]
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[[Category: Bertozzi CR]]
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[[Category: Bertozzi, C R.]]
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[[Category: Leary JA]]
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[[Category: Leary, J A.]]
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[[Category: Lee DH]]
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[[Category: Lee, D H.]]
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[[Category: Lin FL]]
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[[Category: Lin, F L.]]
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[[Category: Mougous JD]]
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[[Category: Mougous, J D.]]
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[[Category: Munchel SE]]
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[[Category: Munchel, S E.]]
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[[Category: Petzold CJ]]
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[[Category: Petzold, C J.]]
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[[Category: Pratt MR]]
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[[Category: Pratt, M R.]]
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[[Category: Riley LW]]
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[[Category: Riley, L W.]]
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[[Category: Senaratne RH]]
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[[Category: Senaratne, R H.]]
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[[Category: TRE]]
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[[Category: mycobacterium]]
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[[Category: sulfation]]
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[[Category: sulfolipid]]
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[[Category: sulfotransferase]]
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[[Category: trehalose]]
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[[Category: trehalose-2-sulfate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:12:47 2008''
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Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose

PDB ID 1tex

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