1rwd

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[[Image:1rwd.png|left|200px]]
 
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{{STRUCTURE_1rwd| PDB=1rwd | SCENE= }}
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==Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings==
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<StructureSection load='1rwd' size='340' side='right'caption='[[1rwd]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rwd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1m2y 1m2y]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RWD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwd OCA], [https://pdbe.org/1rwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rwd RCSB], [https://www.ebi.ac.uk/pdbsum/1rwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rwd ProSAT], [https://www.topsan.org/Proteins/SECSG/1rwd TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RUBR_PYRFU RUBR_PYRFU] Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rw/1rwd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rwd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A new approach for simultaneous protein backbone resonance assignment and structure determination by NMR is introduced. This approach relies on recent advances in high-resolution NMR spectroscopy that allow observation of anisotropic interactions, such as dipolar couplings, from proteins partially aligned in field ordered media. Residual dipolar couplings are used for both geometric information and a filter in the assembly of residues in a sequential manner. Experimental data were collected in less than one week on a small redox protein, rubredoxin, that was 15N enriched but not enriched above 1% natural abundance in 13C. Given the acceleration possible with partial 13C enrichment, the protocol described should provide a very rapid route to protein structure determination. This is critical for the structural genomics initiative where protein expression and structural determination in a high-throughput manner will be needed.
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===Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings===
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A dipolar coupling based strategy for simultaneous resonance assignment and structure determination of protein backbones.,Tian F, Valafar H, Prestegard JH J Am Chem Soc. 2001 Nov 28;123(47):11791-6. PMID:11716736<ref>PMID:11716736</ref>
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{{ABSTRACT_PUBMED_11716736}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1rwd" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[1rwd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1m2y 1m2y]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWD OCA].
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*[[Rubredoxin 3D structures|Rubredoxin 3D structures]]
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*[[Rubredoxin PDB structures|Rubredoxin PDB structures]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:011716736</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
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[[Category: Prestegard, J H.]]
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[[Category: Prestegard JH]]
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[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]
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[[Category: Tian F]]
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[[Category: Tian, F.]]
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[[Category: Valafar H]]
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[[Category: Valafar, H.]]
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[[Category: Electron transport]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Residual dipolar coupling]]
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[[Category: Secsg]]
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[[Category: Southeast collaboratory for structural genomic]]
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[[Category: Structural genomic]]
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Current revision

Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings

PDB ID 1rwd

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