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1tis

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[[Image:1tis.jpg|left|200px]]<br /><applet load="1tis" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tis, resolution 2.7&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THYMIDYLATE SYNTHASE FROM T4 PHAGE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THYMIDYLATE SYNTHASE FROM T4 PHAGE==
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Thymidylate synthase from phage T4 (T4TS) is part of a complex of several enzymes required for coordinate DNA synthesis in infected Escherichia coli cells. It has been proposed that similar complexes of enzymes related to DNA synthesis are also functional in eukaryotes [Pardee, A. B. (1989) Science 246, 603-608]. To delineate the role of structure in the function of this complex, we have solved the structure of T4TS as a basis for mapping the complex by mutagenesis. The 3.1 A structure of the unliganded enzyme was determined by molecular replacement and refined to 19.9% for all data. Three inserts and one deletion in the coding region are unique to T4TS, and all sites lie on one side of the enzyme surface, possibly encoding unique T4 specific intermolecular interactions during the infective cycle. The crystal structure is generally in the open, unliganded conformation seen in unliganded E. coli TS, as opposed to the closed, ternary complex conformation, except that the critically important C-terminus is inserted into the active site hydrogen bonded to residue Asn85, as seen in functional ternary complex structures. Other differences between E. coli TS and T4TS appear to explain the enhanced binding of folyl polyglutamate to the latter.
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<StructureSection load='1tis' size='340' side='right'caption='[[1tis]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tis]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TIS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tis OCA], [https://pdbe.org/1tis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tis RCSB], [https://www.ebi.ac.uk/pdbsum/1tis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tis ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TYSY_BPT4 TYSY_BPT4]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ti/1tis_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tis ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TIS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIS OCA].
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*[[Thymidylate synthase 3D structures|Thymidylate synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of thymidylate synthase from T4 phage: component of a deoxynucleoside triphosphate-synthesizing complex., Finer-Moore JS, Maley GF, Maley F, Montfort WR, Stroud RM, Biochemistry. 1994 Dec 27;33(51):15459-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7803410 7803410]
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[[Category: Escherichia virus T4]]
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[[Category: Bacteriophage t4]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Finer-Moore J]]
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[[Category: Thymidylate synthase]]
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[[Category: Stroud R]]
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[[Category: Finer-Moore, J.]]
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[[Category: Stroud, R.]]
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[[Category: PO4]]
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[[Category: transferase(methyltransferase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:57 2008''
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Current revision

CRYSTAL STRUCTURE OF THYMIDYLATE SYNTHASE FROM T4 PHAGE

PDB ID 1tis

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