This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1skm
From Proteopedia
(Difference between revisions)
| (7 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | [[Image:1skm.png|left|200px]] | ||
| - | + | ==HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site== | |
| + | <StructureSection load='1skm' size='340' side='right'caption='[[1skm]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1skm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SKM FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HCX:(SOUTH)+BICYCLO[3.1.0]HEXANE'>HCX</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1skm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1skm OCA], [https://pdbe.org/1skm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1skm RCSB], [https://www.ebi.ac.uk/pdbsum/1skm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1skm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sk/1skm_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1skm ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| - | == | + | ==See Also== |
| - | + | *[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]] | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | [[ | + | |
| - | + | ||
| - | + | ||
| - | < | + | |
[[Category: Haemophilus haemolyticus]] | [[Category: Haemophilus haemolyticus]] | ||
| - | [[Category: Banavali | + | [[Category: Large Structures]] |
| - | [[Category: Cheng | + | [[Category: Banavali N]] |
| - | [[Category: Horton | + | [[Category: Cheng X]] |
| - | [[Category: Huang | + | [[Category: Horton JR]] |
| - | [[Category: MacKerell | + | [[Category: Huang N]] |
| - | [[Category: Marquez | + | [[Category: MacKerell AD]] |
| - | [[Category: Ratner | + | [[Category: Marquez VE]] |
| - | + | [[Category: Ratner G]] | |
| - | + | ||
Current revision
HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site
| |||||||||||
