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1tjc

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[[Image:1tjc.gif|left|200px]]<br /><applet load="1tjc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tjc, resolution 2.30&Aring;" />
 
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'''Crystal structure of peptide-substrate-binding domain of human type I collagen prolyl 4-hydroxylase'''<br />
 
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==Overview==
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==Crystal structure of peptide-substrate-binding domain of human type I collagen prolyl 4-hydroxylase==
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Collagen prolyl 4-hydroxylases catalyze the formation of 4-hydroxyproline in -X-Pro-Gly-sequences and have an essential role in collagen synthesis. The vertebrate enzymes are alpha2beta2 tetramers in which the catalytic alpha-subunits contain separate peptide-substrate-binding and catalytic domains. We report on the crystal structure of the peptide-substrate-binding domain of the human type I enzyme refined at 2.3 A resolution. It was found to belong to a family of tetratricopeptide repeat domains that are involved in many protein-protein interactions and consist of five alpha-helices forming two tetratricopeptide repeat motifs plus the solvating helix. A prominent feature of its concave surface is a deep groove lined by tyrosines, a putative binding site for proline-rich Tripeptides. Solvent-exposed side chains of three of the tyrosines have a repeat distance similar to that of a poly-L-proline type II helix. The aromatic surface ends at one of the tyrosines, where the groove curves almost 90 degrees away from the linear arrangement of the three tyrosine side chains, possibly inducing a bent conformation in the bound peptide. This finding is consistent with previous suggestions by others that a minimal structural requirement for proline 4-hydroxylation may be a sequence in the poly-L-proline type II conformation followed by a beta-turn in the Pro-Gly segment. Site-directed mutagenesis indicated that none of the tyrosines was critical for tetramer assembly, whereas most of them were critical for the binding of a peptide substrate and inhibitor both to the domain and the alpha2beta2 enzyme tetramer.
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<StructureSection load='1tjc' size='340' side='right'caption='[[1tjc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tjc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjc OCA], [https://pdbe.org/1tjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tjc RCSB], [https://www.ebi.ac.uk/pdbsum/1tjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tjc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P4HA1_HUMAN P4HA1_HUMAN] Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tjc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tjc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Procollagen-proline_dioxygenase Procollagen-proline dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.11.2 1.14.11.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJC OCA].
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*[[Hydroxylases 3D structures|Hydroxylases 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The peptide-substrate-binding domain of collagen prolyl 4-hydroxylases is a tetratricopeptide repeat domain with functional aromatic residues., Pekkala M, Hieta R, Bergmann U, Kivirikko KI, Wierenga RK, Myllyharju J, J Biol Chem. 2004 Dec 10;279(50):52255-61. Epub 2004 Sep 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15456751 15456751]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Procollagen-proline dioxygenase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Bergmann U]]
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[[Category: Bergmann, U.]]
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[[Category: Hieta R]]
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[[Category: Hieta, R.]]
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[[Category: Kivirikko KI]]
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[[Category: Kivirikko, K I.]]
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[[Category: Myllyharju J]]
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[[Category: Myllyharju, J.]]
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[[Category: Pekkala M]]
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[[Category: Pekkala, M.]]
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[[Category: Wierenga RK]]
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[[Category: Wierenga, R K.]]
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[[Category: helical bundle]]
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[[Category: tpr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:14:06 2008''
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Current revision

Crystal structure of peptide-substrate-binding domain of human type I collagen prolyl 4-hydroxylase

PDB ID 1tjc

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