1r0o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1r0o" [edit=sysop:move=sysop])
Current revision (05:58, 23 August 2023) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1r0o.png|left|200px]]
 
-
{{STRUCTURE_1r0o| PDB=1r0o | SCENE= }}
+
==Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex==
 +
<StructureSection load='1r0o' size='340' side='right'caption='[[1r0o]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1r0o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R0O FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r0o OCA], [https://pdbe.org/1r0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r0o RCSB], [https://www.ebi.ac.uk/pdbsum/1r0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r0o ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/USP_DROME USP_DROME] Receptor for ecdysone. May be an important modulator of insect metamorphosis. Plays an important part in embryonic and post-embryonic development. Binds to ecdysone response elements (ECRES) such as in the promoter region of s15 chorion gene.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/1r0o_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r0o ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Ecdysteroids initiate molting and metamorphosis in insects via a heterodimeric receptor consisting of the ecdysone receptor (EcR) and ultraspiracle (USP). The EcR-USP heterodimer preferentially mediates transcription through highly degenerate pseudo-palindromic response elements, resembling inverted repeats of 5'-AGGTCA-3' separated by 1 bp (IR-1). The requirement for a heterodimeric arrangement of EcR-USP subunits to bind to a symmetric DNA is unusual within the nuclear receptor superfamily. We describe the 2.24 A structure of the EcR-USP DNA-binding domain (DBD) heterodimer bound to an idealized IR-1 element. EcR and USP use similar surfaces, and rely on the deformed minor groove of the DNA to establish protein-protein contacts. As retinoid X receptor (RXR) is the mammalian homolog of USP, we also solved the 2.60 A crystal structure of the EcR-RXR DBD heterodimer on IR-1 and found the dimerization and DNA-binding interfaces to be the same as in the EcR-USP complex. Sequence alignments indicate that the EcR-RXR heterodimer is an important model for understanding how the FXR-RXR heterodimer binds to IR-1 sites.
-
===Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex===
+
Structure of the heterodimeric ecdysone receptor DNA-binding complex.,Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F EMBO J. 2003 Nov 3;22(21):5827-40. PMID:14592980<ref>PMID:14592980</ref>
-
{{ABSTRACT_PUBMED_14592980}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1r0o" style="background-color:#fffaf0;"></div>
-
[[1r0o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0O OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:014592980</ref><references group="xtra"/>
+
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
-
[[Category: Devarakonda, S.]]
+
[[Category: Large Structures]]
-
[[Category: Harp, J M.]]
+
[[Category: Devarakonda S]]
-
[[Category: Kim, Y.]]
+
[[Category: Harp JM]]
-
[[Category: Ozyhar, A.]]
+
[[Category: Kim Y]]
-
[[Category: Rastinejad, F.]]
+
[[Category: Ozyhar A]]
-
[[Category: Dna binding domain]]
+
[[Category: Rastinejad F]]
-
[[Category: Ecdsyone receptor]]
+
-
[[Category: Nuclear receptor]]
+
-
[[Category: Transcription-dna complex]]
+
-
[[Category: Ultraspiracle]]
+

Current revision

Crystal Structure of the Heterodimeric Ecdysone Receptor DNA-binding Complex

PDB ID 1r0o

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1r0o"
Personal tools