1se9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:08, 22 May 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1se9.png|left|200px]]
 
-
{{STRUCTURE_1se9| PDB=1se9 | SCENE= }}
+
==Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana==
 +
<StructureSection load='1se9' size='340' side='right'caption='[[1se9]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1se9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SE9 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1se9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1se9 OCA], [https://pdbe.org/1se9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1se9 RCSB], [https://www.ebi.ac.uk/pdbsum/1se9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1se9 ProSAT], [https://www.topsan.org/Proteins/CESG/1se9 TOPSAN]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MUB1_ARATH MUB1_ARATH] May serve as docking site to facilitate the association of other proteins to the plasma membrane.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/se/1se9_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1se9 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Structural proteomics requires robust, scalable methods. Here we describe a wheat germ cell-free platform for protein production that supports efficient NMR structural studies of eukaryotic proteins and offers advantages over cell-based methods. To illustrate this platform, we describe its application to a specific target (At3g01050.1) from Arabidopsis thaliana. After cloning the target gene into a specialized plasmid, we carry out a small-scale (50 mul) in vitro sequential transcription and translation trial to ascertain the level of protein production and solubility. Next, we prepare mRNA for use in a 4-ml semicontinuous cell-free translation reaction to incorporate (15)N-labeled amino acids into a protein sample that we purify and test for suitability for NMR structural analysis. We then repeat the cell-free approach with (13)C,(15)N-labeled amino acids to prepare a doubly labeled sample. The three-dimensional (3D) structure of At3g01050.1 shows that this protein is an unusual member of the beta-grasp protein family.
-
===Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana===
+
Cell-free protein production and labeling protocol for NMR-based structural proteomics.,Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL Nat Methods. 2004 Nov;1(2):149-53. Epub 2004 Oct 21. PMID:15782178<ref>PMID:15782178</ref>
-
{{ABSTRACT_PUBMED_15782178}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1se9" style="background-color:#fffaf0;"></div>
-
[[1se9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE9 OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:015782178</ref><references group="xtra"/>
+
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
-
[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
+
[[Category: Large Structures]]
-
[[Category: Lytle, B L.]]
+
[[Category: Lytle BL]]
-
[[Category: Peterson, F C.]]
+
[[Category: Peterson FC]]
-
[[Category: Volkman, B F.]]
+
[[Category: Volkman BF]]
-
[[Category: Cell-free]]
+
-
[[Category: Center for eukaryotic structural genomic]]
+
-
[[Category: Cesg]]
+
-
[[Category: Plant protein]]
+
-
[[Category: Protein structure initiative]]
+
-
[[Category: Psi]]
+
-
[[Category: Structural genomic]]
+
-
[[Category: Ubiquitin-like]]
+
-
[[Category: Wheat germ]]
+

Current revision

Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana

PDB ID 1se9

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1se9"
Personal tools