1ri5

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[[Image:1ri5.png|left|200px]]
 
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{{STRUCTURE_1ri5| PDB=1ri5 | SCENE= }}
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==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==
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<StructureSection load='1ri5' size='340' side='right'caption='[[1ri5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ri5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RI5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ri5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri5 OCA], [https://pdbe.org/1ri5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ri5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ri5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri5 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1ri5 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1ri5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ri5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.
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===Structure and mechanism of mRNA cap (guanine N-7) methyltransferase===
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Structure and mechanism of mRNA cap (guanine-N7) methyltransferase.,Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD Mol Cell. 2004 Jan 16;13(1):77-89. PMID:14731396<ref>PMID:14731396</ref>
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{{ABSTRACT_PUBMED_14731396}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1ri5" style="background-color:#fffaf0;"></div>
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[[1ri5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI5 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:014731396</ref><references group="xtra"/>
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</StructureSection>
[[Category: Encephalitozoon cuniculi]]
[[Category: Encephalitozoon cuniculi]]
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[[Category: Burley, S K.]]
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[[Category: Large Structures]]
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[[Category: Fabrega, C.]]
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[[Category: Burley SK]]
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[[Category: Hausmann, S.]]
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[[Category: Fabrega C]]
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[[Category: Lima, C D.]]
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[[Category: Hausmann S]]
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[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics.]]
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[[Category: Lima CD]]
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[[Category: Shen, V.]]
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[[Category: Shen V]]
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[[Category: Shuman, S.]]
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[[Category: Shuman S]]
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[[Category: Cap]]
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[[Category: M7g]]
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[[Category: Messenger rna cap]]
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[[Category: Methyltransferase]]
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[[Category: New york sgx research center for structural genomic]]
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[[Category: Nysgxrc]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Rna]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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Current revision

Structure and mechanism of mRNA cap (guanine N-7) methyltransferase

PDB ID 1ri5

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