1sl7

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[[Image:1sl7.png|left|200px]]
 
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{{STRUCTURE_1sl7| PDB=1sl7 | SCENE= }}
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==Crystal structure of calcium-loaded apo-obelin from Obelia longissima==
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<StructureSection load='1sl7' size='340' side='right'caption='[[1sl7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sl7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SL7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sl7 OCA], [https://pdbe.org/1sl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sl7 RCSB], [https://www.ebi.ac.uk/pdbsum/1sl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sl7 ProSAT], [https://www.topsan.org/Proteins/SECSG/1sl7 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OBL_OBELO OBL_OBELO] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/1sl7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sl7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structures of calcium-loaded apo-aequorin and apo-obelin have been determined at resolutions 1.7A and 2.2 A, respectively. A calcium ion is observed in each of the three EF-hand loops that have the canonical calcium-binding sequence, and each is coordinated in the characteristic pentagonal bipyramidal configuration. The calcium-loaded apo-protein retain the same compact scaffold and overall fold as the unreacted photoproteins containing the bound substrate, 2-hyroperoxycoelenterazine, and also the same as the Ca2+-discharged obelin bound with product, coleneteramide. Nevertheless, there are easily discerned shifts in both helix and loop regions, and the shifts are not the same between the two proteins. It is suggested that these photoproteins to sense Ca2+ concentration transients and to produce their bioluminescence response on the millisecond timescale. A mechanism of intrastructural transmission of the calcium signal is proposed.
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===Crystal structure of calcium-loaded apo-obelin from Obelia longissima===
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All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin.,Deng L, Vysotski ES, Markova SV, Liu ZJ, Lee J, Rose J, Wang BC Protein Sci. 2005 Mar;14(3):663-75. Epub 2005 Feb 2. PMID:15689515<ref>PMID:15689515</ref>
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{{ABSTRACT_PUBMED_15689515}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1sl7" style="background-color:#fffaf0;"></div>
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[[1sl7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SL7 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015689515</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Obelia longissima]]
[[Category: Obelia longissima]]
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[[Category: Deng, L.]]
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[[Category: Deng L]]
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[[Category: Lee, J.]]
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[[Category: Lee J]]
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[[Category: Liu, Z J.]]
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[[Category: Liu ZJ]]
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[[Category: Markova, S V.]]
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[[Category: Markova SV]]
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[[Category: Rose, J.]]
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[[Category: Rose J]]
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[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]
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[[Category: Vysotski ES]]
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[[Category: Vysotski, E S.]]
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[[Category: Wang BC]]
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[[Category: Wang, B C.]]
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[[Category: Aequorin]]
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[[Category: Bioluminescence]]
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[[Category: Calcium binding]]
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[[Category: Ef-hand]]
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[[Category: Fluorescence]]
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[[Category: Luminescent protein]]
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[[Category: Obelin]]
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[[Category: Photoprotein]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Secsg]]
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[[Category: Southeast collaboratory for structural genomic]]
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[[Category: Structural genomic]]
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Current revision

Crystal structure of calcium-loaded apo-obelin from Obelia longissima

PDB ID 1sl7

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