1sr4

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[[Image:1sr4.png|left|200px]]
 
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{{STRUCTURE_1sr4| PDB=1sr4 | SCENE= }}
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==Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin==
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<StructureSection load='1sr4' size='340' side='right'caption='[[1sr4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [https://pdbe.org/1sr4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [https://www.ebi.ac.uk/pdbsum/1sr4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDTA_HAEDU CDTA_HAEDU] CDTs are cytotoxins which induce host cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells. CdtA, along with CdtC, probably forms a heterodimeric subunit required for the delivery of CdtB.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sr4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sr4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The tripartite cytolethal distending toxin (CDT) induces cell cycle arrest and apoptosis in eukaryotic cells. The subunits CdtA and CdtC associate with the nuclease CdtB to form a holotoxin that translocates CdtB into the host cell, where it acts as a genotoxin by creating DNA lesions. Here we show that the crystal structure of the holotoxin from Haemophilus ducreyi reveals that CDT consists of an enzyme of the DNase-I family, bound to two ricin-like lectin domains. CdtA, CdtB and CdtC form a ternary complex with three interdependent molecular interfaces, characterized by globular, as well as extensive non-globular, interactions. The lectin subunits form a deeply grooved, highly aromatic surface that we show to be critical for toxicity. The holotoxin possesses a steric block of the CdtB active site by means of a non-globular extension of the CdtC subunit, and we identify putative DNA binding residues in CdtB that are essential for toxin activity.
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===Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin===
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Assembly and function of a bacterial genotoxin.,Nesic D, Hsu Y, Stebbins CE Nature. 2004 May 27;429(6990):429-33. PMID:15164065<ref>PMID:15164065</ref>
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{{ABSTRACT_PUBMED_15164065}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1sr4" style="background-color:#fffaf0;"></div>
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[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015164065</ref><ref group="xtra">PMID:017009862</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Haemophilus ducreyi]]
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[[Category: Large Structures]]
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[[Category: Hsu, Y.]]
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[[Category: Hsu Y]]
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[[Category: Nesic, D.]]
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[[Category: Nesic D]]
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[[Category: Stebbins, C E.]]
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[[Category: Stebbins CE]]
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[[Category: Apoptosis]]
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[[Category: Bacterial]]
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[[Category: Cell cycle]]
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[[Category: Cytotoxin]]
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[[Category: Deoxyribonuclease i]]
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[[Category: Dna damage]]
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[[Category: Genotoxin]]
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[[Category: Haemophilus ducreyi]]
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[[Category: Lectin]]
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[[Category: Toxin]]
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[[Category: Virulence]]
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Current revision

Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin

PDB ID 1sr4

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