1ry4

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[[Image:1ry4.png|left|200px]]
 
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{{STRUCTURE_1ry4| PDB=1ry4 | SCENE= }}
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==NMR Structure of the CRIB-PDZ module of Par-6==
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<StructureSection load='1ry4' size='340' side='right'caption='[[1ry4]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ry4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RY4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ry4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ry4 OCA], [https://pdbe.org/1ry4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ry4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ry4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ry4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O97111_DROME O97111_DROME]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ry/1ry4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ry4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Regulation of protein interaction domains is required for cellular signaling dynamics. Here, we show that the PDZ protein interaction domain from the cell polarity protein Par-6 is regulated by the Rho GTPase Cdc42. Cdc42 binds to a CRIB domain adjacent to the PDZ domain, increasing the affinity of the Par-6 PDZ for its carboxy-terminal ligand by approximately 13-fold. Par-6 PDZ regulation is required for function as mutational disruption of Cdc42-Par-6 PDZ coupling leads to inactivation of Par-6 in polarized MDCK epithelial cells. Structural analysis reveals that the free PDZ domain has several deviations from the canonical PDZ conformation that account for its low ligand affinity. Regulation results from a Cdc42-induced conformational transition in the CRIB-PDZ module that causes the PDZ to assume a canonical, high-affinity PDZ conformation. The coupled CRIB and PDZ architecture of Par-6 reveals how simple binding domains can be combined to yield complex regulation.
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===NMR Structure of the CRIB-PDZ module of Par-6===
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Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition.,Peterson FC, Penkert RR, Volkman BF, Prehoda KE Mol Cell. 2004 Mar 12;13(5):665-76. PMID:15023337<ref>PMID:15023337</ref>
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{{ABSTRACT_PUBMED_15023337}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1ry4" style="background-color:#fffaf0;"></div>
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[[1ry4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY4 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015023337</ref><references group="xtra"/>
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Penkert, R R.]]
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[[Category: Large Structures]]
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[[Category: Peterson, F C.]]
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[[Category: Penkert RR]]
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[[Category: Prehoda, K E.]]
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[[Category: Peterson FC]]
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[[Category: Volkman, B F.]]
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[[Category: Prehoda KE]]
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[[Category: Cdc-42]]
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[[Category: Volkman BF]]
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[[Category: Cell adhesion]]
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[[Category: Cell polarization]]
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[[Category: Crib]]
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[[Category: Pdz]]
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[[Category: Polarity adaptor complex]]
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Current revision

NMR Structure of the CRIB-PDZ module of Par-6

PDB ID 1ry4

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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