1u0w

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[[Image:1u0w.png|left|200px]]
 
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{{STRUCTURE_1u0w| PDB=1u0w | SCENE= }}
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==An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: 18xCHS+resveratrol Structure==
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<StructureSection load='1u0w' size='340' side='right'caption='[[1u0w]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u0w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=STL:RESVERATROL'>STL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0w OCA], [https://pdbe.org/1u0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0w RCSB], [https://www.ebi.ac.uk/pdbsum/1u0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHS2_MEDSA CHS2_MEDSA] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.<ref>PMID:10653632</ref> <ref>PMID:11732902</ref> <ref>PMID:11959984</ref> <ref>PMID:15380179</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Stilbene synthase (STS) and chalcone synthase (CHS) each catalyze the formation of a tetraketide intermediate from a CoA-tethered phenylpropanoid starter and three molecules of malonyl-CoA, but use different cyclization mechanisms to produce distinct chemical scaffolds for a variety of plant natural products. Here we present the first STS crystal structure and identify, by mutagenic conversion of alfalfa CHS into a functional stilbene synthase, the structural basis for the evolution of STS cyclization specificity in type III polyketide synthase (PKS) enzymes. Additional mutagenesis and enzymatic characterization confirms that electronic effects rather than steric factors balance competing cyclization specificities in CHS and STS. Finally, we discuss the problematic in vitro reconstitution of plant stilbenecarboxylate pathways, using insights from existing biomimetic polyketide cyclization studies to generate a novel mechanistic hypothesis to explain stilbenecarboxylate biosynthesis.
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===An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: 18xCHS+resveratrol Structure===
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An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases.,Austin MB, Bowman ME, Ferrer JL, Schroder J, Noel JP Chem Biol. 2004 Sep;11(9):1179-94. PMID:15380179<ref>PMID:15380179</ref>
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{{ABSTRACT_PUBMED_15380179}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1u0w" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[1u0w]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0W OCA].
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*[[Chalcone synthase|Chalcone synthase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015380179</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Medicago sativa]]
[[Category: Medicago sativa]]
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[[Category: Naringenin-chalcone synthase]]
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[[Category: Austin MB]]
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[[Category: Austin, M B.]]
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[[Category: Bowman ME]]
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[[Category: Bowman, M E.]]
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[[Category: Ferrer J-L]]
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[[Category: Ferrer, J L.]]
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[[Category: Noel JP]]
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[[Category: Noel, J P.]]
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[[Category: Schroder J]]
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[[Category: Schroder, J.]]
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[[Category: Aldol switch]]
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[[Category: Alpha-beta-alpha-beta-alpha fold]]
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[[Category: Catalytic triad]]
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[[Category: Chalcone synthase]]
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[[Category: Ch]]
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[[Category: Condensing enzyme]]
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[[Category: Engineered resveratrol synthase]]
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[[Category: Pk]]
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[[Category: R]]
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[[Category: Stilbene synthase]]
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[[Category: St]]
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[[Category: Thiolase fold]]
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[[Category: Transferase]]
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[[Category: Type iii polyketide synthase]]
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Current revision

An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: 18xCHS+resveratrol Structure

PDB ID 1u0w

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