This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1fwj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:17, 27 March 2024) (edit) (undo)
 
(20 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1fwj.gif|left|200px]]<br />
 
-
<applet load="1fwj" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1fwj, resolution 2.2&Aring;" />
 
-
'''KLEBSIELLA AEROGENES UREASE, NATIVE'''<br />
 
-
==Overview==
+
==KLEBSIELLA AEROGENES UREASE, NATIVE==
-
Cys319 is located on a mobile flap covering the active site of Klebsiella, aerogenes urease but does not play an essential role in catalysis. Four, urease variants altered at position C319 range from having high activity, (C319A) to no measurable activity (C319Y), indicating Cys is not required, at this position, but its presence is highly influential [Martin, P. R., &amp;, Hausinger, R. P. (1992) J. Biol. Chem. 267, 20024-20027]. Here, we present, 2.0 A resolution crystal structures of C319A, C319S, C319D, and C319Y, proteins and the C319A variant inhibited by acetohydroxamic acid. These, structures show changes in the hydration of the active site nickel ions, and in the position and flexibility of the active site flap. The C319Y, protein exhibits an alternate conformation of the flap, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9201965 (full description)]]
+
<StructureSection load='1fwj' size='340' side='right'caption='[[1fwj]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1fwj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1kau 1kau]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FWJ FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fwj OCA], [https://pdbe.org/1fwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fwj RCSB], [https://www.ebi.ac.uk/pdbsum/1fwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fwj ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/URE3_KLEAE URE3_KLEAE]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/1fwj_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fwj ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1FWJ is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with NI as [[http://en.wikipedia.org/wiki/ligand ligand]]. This structure superseeds the now removed PDB entry 1KAU. Active as [[http://en.wikipedia.org/wiki/Urease Urease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5]]. Structure known Active Sites: ACT and NIL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FWJ OCA]].
+
*[[Urease 3D structures|Urease 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structures of Cys319 variants and acetohydroxamate-inhibited Klebsiella aerogenes urease., Pearson MA, Michel LO, Hausinger RP, Karplus PA, Biochemistry. 1997 Jul 1;36(26):8164-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9201965 9201965]
+
[[Category: Klebsiella aerogenes]]
[[Category: Klebsiella aerogenes]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Urease]]
+
[[Category: Karplus PA]]
-
[[Category: Karplus, P.A.]]
+
[[Category: Pearson MA]]
-
[[Category: Pearson, M.A.]]
+
-
[[Category: NI]]
+
-
[[Category: hydrolase(urea amido)]]
+
-
[[Category: nickel metalloenzyme]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:13:20 2007''
+

Current revision

KLEBSIELLA AEROGENES UREASE, NATIVE

PDB ID 1fwj

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fwj"
Personal tools