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1txb

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[[Image:1txb.gif|left|200px]]<br /><applet load="1txb" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1txb" />
 
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'''SOLUTION NMR STRUCTURE OF TOXIN B, A LONG NEUROTOXIN FROM THE VENOM OF THE KING COBRA, 10 STRUCTURES'''<br />
 
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==Overview==
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==SOLUTION NMR STRUCTURE OF TOXIN B, A LONG NEUROTOXIN FROM THE VENOM OF THE KING COBRA, 10 STRUCTURES==
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The solution structure of toxin b, a long neurotoxin (73 amino acids and 5 disulfides) from the venom of Ophiophagus hannah (king cobra), has been determined using 1H NMR and dynamical simulated annealing techniques. The structures were calculated using 485 distance constraints and 52 dihedral angle restraints. The 21 structures that were obtained satisfy the experimental restraints and possess good nonbonded contacts. Analysis of the converged structures revealed that the protein consists of a core region from which three finger-like loops extend outwards. The regular secondary structure in toxin b includes a double and a triple stranded antiparallel beta sheet. Comparison with the solution structures of other long neurotoxins reveals that although the structure of toxin b is similar to those of previously reported long neurotoxins, clear local structural differences are observed in regions proposed to be involved in binding to the acetylcholine receptor. A positively charged cluster is found in the C-terminal tail, in Loop III, and in the tip of Loop II. This cationic cluster could be crucial for the binding of the long neurotoxins to the acetylcholine receptor.
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<StructureSection load='1txb' size='340' side='right'caption='[[1txb]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1txb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ophiophagus_hannah Ophiophagus hannah]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXB FirstGlance]. <br>
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1TXB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ophiophagus_hannah Ophiophagus hannah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txb OCA], [https://pdbe.org/1txb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txb RCSB], [https://www.ebi.ac.uk/pdbsum/1txb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txb ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of toxin b, a long neurotoxin from the venom of the king cobra (Ophiophagus hannah)., Peng SS, Kumar TK, Jayaraman G, Chang CC, Yu C, J Biol Chem. 1997 Mar 21;272(12):7817-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9065446 9065446]
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== Function ==
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[https://www.uniprot.org/uniprot/3L22_OPHHA 3L22_OPHHA] Binds with high affinity to muscular (alpha-1/CHRNA1) and neuronal (alpha-7/CHRNA7) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission.[UniProtKB:P60615]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txb ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Ophiophagus hannah]]
[[Category: Ophiophagus hannah]]
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[[Category: Single protein]]
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[[Category: Chang C-C]]
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[[Category: Chang, C C.]]
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[[Category: Jayaraman G]]
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[[Category: Jayaraman, G.]]
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[[Category: Kumar TKS]]
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[[Category: Kumar, T K.S.]]
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[[Category: Peng S-S]]
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[[Category: Peng, S S.]]
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[[Category: Yu C]]
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[[Category: Yu, C.]]
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[[Category: multigene family]]
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[[Category: neurotoxin]]
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[[Category: toxin b]]
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[[Category: venom]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:18:21 2008''
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Current revision

SOLUTION NMR STRUCTURE OF TOXIN B, A LONG NEUROTOXIN FROM THE VENOM OF THE KING COBRA, 10 STRUCTURES

PDB ID 1txb

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