1wij

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[[Image:1wij.png|left|200px]]
 
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{{STRUCTURE_1wij| PDB=1wij | SCENE= }}
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==Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3==
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<StructureSection load='1wij' size='340' side='right'caption='[[1wij]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wij]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WIJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wij OCA], [https://pdbe.org/1wij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wij RCSB], [https://www.ebi.ac.uk/pdbsum/1wij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wij ProSAT], [https://www.topsan.org/Proteins/RSGI/1wij TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EIL3_ARATH EIL3_ARATH] Probable transcription factor that may be involved in the ethylene response pathway.<ref>PMID:9215635</ref> <ref>PMID:9851977</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wi/1wij_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wij ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential transcription factors in the ethylene signaling of higher plants. The EIN3/EIL proteins bind to the promoter regions of the downstream genes and regulate their expression. The location of the DNA-binding domain (DBD) in the primary structure was unclear, since the proteins show no sequence similarity to other known DBDs. Here, we identify the major DBD of an EIN3/EIL protein, Arabidopsis thaliana EIL3, containing a key mutational site for DNA binding and signaling (ein3-3 site), and determine its solution structure by NMR spectroscopy. The structure consists of five alpha-helices, possessing a novel fold dissimilar to known DBD structures. By a chemical-shift perturbation analysis, a region including the ein3-3 site is suggested to be involved in DNA binding.
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===Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3===
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Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3.,Yamasaki K, Kigawa T, Inoue M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S J Mol Biol. 2005 Apr 29;348(2):253-64. PMID:15811366<ref>PMID:15811366</ref>
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{{ABSTRACT_PUBMED_15811366}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1wij" style="background-color:#fffaf0;"></div>
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[[1wij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WIJ OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015811366</ref><references group="xtra"/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Inoue, M.]]
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[[Category: Large Structures]]
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[[Category: Kigawa, T.]]
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[[Category: Inoue M]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Kigawa T]]
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[[Category: Yamasaki, K.]]
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[[Category: Yamasaki K]]
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[[Category: Yokoyama, S.]]
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[[Category: Yokoyama S]]
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[[Category: Dna binding protein]]
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[[Category: Dna-binding domain]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: Rsgi]]
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[[Category: Structural genomic]]
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Current revision

Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3

PDB ID 1wij

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