1xcv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1xcv" [edit=sysop:move=sysop])
Current revision (06:43, 23 August 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1xcv.png|left|200px]]
 
-
{{STRUCTURE_1xcv| PDB=1xcv | SCENE= }}
+
==Crystal Structure Of (H79AC102D)Dtxr complexed with Nickel(II)==
 +
<StructureSection load='1xcv' size='340' side='right'caption='[[1xcv]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1xcv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCV FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcv OCA], [https://pdbe.org/1xcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcv RCSB], [https://www.ebi.ac.uk/pdbsum/1xcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcv ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DTXR_CORDI DTXR_CORDI] Iron-binding repressor of the dipheteria toxin gene expression. May serve as a global regulator of gene expression. Represses ripA under iron excess.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcv_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcv ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The diphtheria toxin repressor (DtxR) is a metal ion-activated transcriptional regulator that has been linked to the virulence of Corynebacterium diphtheriae. Structure determination has shown that there are two metal ion binding sites per repressor monomer, and site-directed mutagenesis has demonstrated that binding site 2 (primary) is essential for recognition of the target DNA repressor, leaving the role of binding site 1 (ancillary) unclear. Calorimetric techniques have demonstrated that although binding site 1 (ancillary) has high affinity for metal ion with a binding constant of 2 x 10(-7), binding site 2 (primary) is a low-affinity binding site with a binding constant of 6.3 x 10(-4). These two binding sites act in an independent fashion, and their contribution can be easily dissected by traditional mutational analysis. Our results clearly demonstrate that binding site 1 (ancillary) is the first one to be occupied during metal ion activation, playing a critical role in stabilization of the repressor. In addition, structural data obtained for the mutants Ni-DtxR(H79A,C102D), reported here, and the previously reported DtxR(H79A) have allowed us to propose a mechanism of metal activation for DtxR.
-
===Crystal Structure Of (H79AC102D)Dtxr complexed with Nickel(II)===
+
Mechanism of metal ion activation of the diphtheria toxin repressor DtxR.,D'Aquino JA, Tetenbaum-Novatt J, White A, Berkovitch F, Ringe D Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18408-13. Epub 2005 Dec 13. PMID:16352732<ref>PMID:16352732</ref>
-
{{ABSTRACT_PUBMED_16352732}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1xcv" style="background-color:#fffaf0;"></div>
-
==About this Structure==
+
==See Also==
-
[[1xcv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCV OCA].
+
*[[Diphtheria toxin repressor|Diphtheria toxin repressor]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:016352732</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Corynebacterium diphtheriae]]
[[Category: Corynebacterium diphtheriae]]
-
[[Category: Ringe, D.]]
+
[[Category: Large Structures]]
-
[[Category: Aquino, J A.D.]]
+
[[Category: D'aquino JA]]
-
[[Category: Dna binding protein]]
+
[[Category: Ringe D]]
-
[[Category: Helix-turn-helix]]
+

Current revision

Crystal Structure Of (H79AC102D)Dtxr complexed with Nickel(II)

PDB ID 1xcv

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xcv"
Personal tools