1u12

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[[Image:1u12.gif|left|200px]]<br /><applet load="1u12" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u12, resolution 2.70&Aring;" />
 
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'''M. loti cyclic nucleotide binding domain mutant'''<br />
 
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==Overview==
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==M. loti cyclic nucleotide binding domain mutant==
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Here we describe the initial functional characterization of a cyclic nucleotide regulated ion channel from the bacterium Mesorhizobium loti and present two structures of its cyclic nucleotide binding domain, with and without cAMP. The domains are organized as dimers with the interface formed by the linker regions that connect the nucleotide binding pocket to the pore domain. Together, structural and functional data suggest the domains form two dimers on the cytoplasmic face of the channel. We propose a model for gating in which ligand binding alters the structural relationship within a dimer, directly affecting the position of the adjacent transmembrane helices.
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<StructureSection load='1u12' size='340' side='right'caption='[[1u12]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u12]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_japonicum_MAFF_303099 Mesorhizobium japonicum MAFF 303099]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U12 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u12 OCA], [https://pdbe.org/1u12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u12 RCSB], [https://www.ebi.ac.uk/pdbsum/1u12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u12 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CNGK1_RHILO CNGK1_RHILO] Cyclic nucleotide-regulated potassium channel activated by cAMP.<ref>PMID:15550244</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u1/1u12_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u12 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1U12 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti_maff303099 Mesorhizobium loti maff303099] with <scene name='pdbligand=IOD:'>IOD</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U12 OCA].
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel., Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH, Cell. 2004 Nov 24;119(5):615-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15550244 15550244]
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__TOC__
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[[Category: Mesorhizobium loti maff303099]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Clayton, G M.]]
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[[Category: Mesorhizobium japonicum MAFF 303099]]
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[[Category: Heginbotham, L.]]
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[[Category: Clayton GM]]
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[[Category: Morais-Cabral, J H.]]
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[[Category: Heginbotham L]]
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[[Category: Silverman, W R.]]
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[[Category: Morais-Cabral JH]]
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[[Category: IOD]]
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[[Category: Silverman WR]]
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[[Category: K]]
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[[Category: SO4]]
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[[Category: c-helix mutation]]
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[[Category: mutant cyclic nucleotide binding domain]]
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[[Category: unliganded]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:29 2008''
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Current revision

M. loti cyclic nucleotide binding domain mutant

PDB ID 1u12

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