1tx9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1tx9" [edit=sysop:move=sysop])
Current revision (06:35, 23 August 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1tx9.png|left|200px]]
 
-
{{STRUCTURE_1tx9| PDB=1tx9 | SCENE= }}
+
==gpd prior to capsid assembly==
 +
<StructureSection load='1tx9' size='340' side='right'caption='[[1tx9]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1tx9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_phiX174 Escherichia virus phiX174]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TX9 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.31&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tx9 OCA], [https://pdbe.org/1tx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tx9 RCSB], [https://www.ebi.ac.uk/pdbsum/1tx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tx9 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SCAFD_BPPHS SCAFD_BPPHS] Assembles the procapsid by joining twelve 12S pre-assembly complex into a T=1 icosahedral particle, called 108S procapsid. Ten proteins D bind each 12S complex, which are formed by three pentamers of F, G, B protein and a H protein. The scaffolding protein is released from the provirion after genome packaging to form the mature virion.<ref>PMID:15890913</ref> <ref>PMID:159449</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1tx9_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tx9 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The three-dimensional structure of bacteriophage phiX174 external scaffolding protein D, prior to its interaction with other structural proteins, has been determined to 3.3 angstroms by X-ray crystallography. The crystals belong to space group P4(1)2(1)2 with a dimer in the asymmetric unit that closely resembles asymmetric dimers observed in the phiX174 procapsid structure. Furthermore, application of the crystallographic 4(1) symmetry operation to one of these dimers generates a tetramer similar to the tetramer in the icosahedral asymmetric unit of the procapsid. These data suggest that both dimers and tetramers of the D protein are true morphogenetic intermediates and can form independently of other proteins involved in procapsid morphogenesis. The crystal structure of the D scaffolding protein thus represents the state of the polypeptide prior to procapsid assembly. Hence, comparison with the procapsid structure provides a rare opportunity to follow the conformational switching events necessary for the construction of complex macromolecular assemblies.
-
===gpd prior to capsid assembly===
+
Conformational switching by the scaffolding protein D directs the assembly of bacteriophage phiX174.,Morais MC, Fisher M, Kanamaru S, Przybyla L, Burgner J, Fane BA, Rossmann MG Mol Cell. 2004 Sep 24;15(6):991-7. PMID:15383287<ref>PMID:15383287</ref>
-
{{ABSTRACT_PUBMED_15383287}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1tx9" style="background-color:#fffaf0;"></div>
-
[[1tx9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_phix174 Enterobacteria phage phix174]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TX9 OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:015383287</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Enterobacteria phage phix174]]
+
[[Category: Escherichia virus phiX174]]
-
[[Category: Fane, B A.]]
+
[[Category: Large Structures]]
-
[[Category: Fisher, M.]]
+
[[Category: Fane BA]]
-
[[Category: Kanamaru, K.]]
+
[[Category: Fisher M]]
-
[[Category: Morais, M C.]]
+
[[Category: Kanamaru K]]
-
[[Category: Rossmann, M G.]]
+
[[Category: Morais MC]]
-
[[Category: Assembly]]
+
[[Category: Rossmann MG]]
-
[[Category: Conformational switching]]
+
-
[[Category: Phix174]]
+
-
[[Category: Scaffolding protein]]
+
-
[[Category: Structural protein]]
+

Current revision

gpd prior to capsid assembly

PDB ID 1tx9

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tx9"
Personal tools