1u5w

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[[Image:1u5w.gif|left|200px]]<br /><applet load="1u5w" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u5w, resolution 2.3&Aring;" />
 
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'''Crystal structure of hypothetical protein yjjX from Escherichia coli'''<br />
 
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==Overview==
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==Crystal structure of hypothetical protein yjjX from Escherichia coli==
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Inosine triphosphate (ITP) and xanthosine triphosphate (XTP) are formed upon deamination of ATP and GTP as a result of exposure to chemical mutagens and oxidative damage. Nucleic acid synthesis requires safeguard mechanisms to minimize undesired lethal incorporation of ITP and XTP. Here, we present the crystal structure of YjjX, a protein of hitherto unknown function. The three-dimensional fold of YjjX is similar to those of Mj0226 from Methanococcus janschii, which possesses nucleotidase activity, and of Maf from Bacillus subtilis, which can bind nucleotides. Biochemical analyses of YjjX revealed it to exhibit specific phosphatase activity for inosine and xanthosine triphosphates and have a possible interaction with elongation factor Tu. The enzymatic activity of YjjX as an inosine/xanthosine triphosphatase provides evidence for a plausible protection mechanism by clearing the noncanonical nucleotides from the cell during oxidative stress in E. coli.
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<StructureSection load='1u5w' size='340' side='right'caption='[[1u5w]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1u5w]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U5W FirstGlance]. <br>
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1U5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5W OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5w OCA], [https://pdbe.org/1u5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u5w RCSB], [https://www.ebi.ac.uk/pdbsum/1u5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u5w ProSAT], [https://www.topsan.org/Proteins/BSGI/1u5w TOPSAN]</span></td></tr>
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Identification of an ITPase/XTPase in Escherichia coli by structural and biochemical analysis., Zheng J, Singh VK, Jia Z, Structure. 2005 Oct;13(10):1511-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16216582 16216582]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCPP_ECOLI NCPP_ECOLI] Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions. ITP is the best substrate, followed by XTP, GDP or dITP. Also implicated in the resistance against the thiamine metabolism inhibitors bacimethrin and CF3-HMP.<ref>PMID:16216582</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u5/1u5w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u5w ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
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[[Category: Jia Z]]
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[[Category: Jia, Z.]]
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[[Category: Singh VK]]
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[[Category: Singh, V K.]]
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[[Category: Zheng J]]
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[[Category: Zheng, J.]]
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[[Category: SO4]]
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[[Category: 3 layers alpha/beta/alpha protein]]
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[[Category: bsgi]]
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[[Category: montreal-kingston bacterial structural genomics initiative]]
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[[Category: structural genomics]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:20:54 2008''
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Current revision

Crystal structure of hypothetical protein yjjX from Escherichia coli

PDB ID 1u5w

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