1u8y

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[[Image:1u8y.jpg|left|200px]]<br /><applet load="1u8y" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u8y, resolution 1.55&Aring;" />
 
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'''CRystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap'''<br />
 
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==Overview==
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==CRystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap==
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RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap.
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<StructureSection load='1u8y' size='340' side='right'caption='[[1u8y]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1u8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saguinus_oedipus Saguinus oedipus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U8Y FirstGlance]. <br>
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1U8Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saguinus_oedipus Saguinus oedipus] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GNP:'>GNP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8Y OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8y OCA], [https://pdbe.org/1u8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u8y RCSB], [https://www.ebi.ac.uk/pdbsum/1u8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u8y ProSAT]</span></td></tr>
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Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15530367 15530367]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RALA_SAGOE RALA_SAGOE] Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u8/1u8y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u8y ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saguinus oedipus]]
[[Category: Saguinus oedipus]]
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[[Category: Single protein]]
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[[Category: Kosak J]]
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[[Category: Kosak, J.]]
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[[Category: Mattos C]]
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[[Category: Mattos, C.]]
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[[Category: Nicely NI]]
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[[Category: Nicely, N I.]]
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[[Category: De Serrano V]]
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[[Category: Serrano, V de.]]
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[[Category: GNP]]
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[[Category: MG]]
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[[Category: signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:21:49 2008''
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Current revision

CRystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap

PDB ID 1u8y

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