1t4x
From Proteopedia
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- | [[Image:1t4x.png|left|200px]] | ||
- | + | ==The first left-handed RNA structure of (CGCGCG)2, Z-RNA, NMR, 12 structures, determined in high salt== | |
+ | <StructureSection load='1t4x' size='340' side='right'caption='[[1t4x]]' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[1t4x]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T4X FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t4x OCA], [https://pdbe.org/1t4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t4x RCSB], [https://www.ebi.ac.uk/pdbsum/1t4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t4x ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Right-handed RNA duplexes of (CG)n sequence undergo salt-induced helicity reversal, forming left-handed RNA double helices (Z-RNA). In contrast to the thoroughly studied Z-DNA, no Z-RNA structure of natural origin is known. Here we report the NMR structure of a half-turn, left-handed RNA helix (CGCGCG)2 determined in 6 M NaClO4. This is the first nucleic acid motif determined at such high salt. Sequential assignments of non-exchangeable proton resonances of the Z-form were based on the hitherto unreported NOE connectivity path [H6(n)-H5'/H5''(n)-H8(n+1)-H1'(n+1)-H6(n+2)] found for left-handed helices. Z-RNA structure shows several conformational features significantly different from Z-DNA. Intra-strand but no inter-strand base stacking was observed for both CpG and GpC steps. Helical twist angles for CpG steps have small positive values (4-7 degrees), whereas GpC steps have large negative values (-61 degrees). In the full-turn model of Z-RNA (12.4 bp per turn), base pairs are much closer to the helix axis than in Z-DNA, thus both the very deep, narrow minor groove with buried cytidine 2'-OH groups, and the major groove are well defined. The 2'-OH group of cytidines plays a crucial role in the Z-RNA structure and its formation; 2'-O-methylation of cytidine, but not of guanosine residues prohibits A to Z helicity reversal. | ||
- | + | High salt solution structure of a left-handed RNA double helix.,Popenda M, Milecki J, Adamiak RW Nucleic Acids Res. 2004 Aug 3;32(13):4044-54. Print 2004. PMID:15292450<ref>PMID:15292450</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | + | </div> | |
- | + | <div class="pdbe-citations 1t4x" style="background-color:#fffaf0;"></div> | |
- | + | == References == | |
- | + | <references/> | |
- | + | __TOC__ | |
- | [[Category: | + | </StructureSection> |
- | [[Category: | + | [[Category: Large Structures]] |
- | [[Category: | + | [[Category: Adamiak RW]] |
- | [[Category: | + | [[Category: Milecki J]] |
+ | [[Category: Popenda M]] |
Current revision
The first left-handed RNA structure of (CGCGCG)2, Z-RNA, NMR, 12 structures, determined in high salt
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