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1ugl
From Proteopedia
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| - | [[Image:1ugl.jpg|left|200px]]<br /><applet load="1ugl" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1ugl" /> | ||
| - | '''Solution structure of S8-SP11'''<br /> | ||
| - | == | + | ==Solution structure of S8-SP11== |
| + | <StructureSection load='1ugl' size='340' side='right'caption='[[1ugl]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1ugl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UGL FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ugl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ugl OCA], [https://pdbe.org/1ugl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ugl RCSB], [https://www.ebi.ac.uk/pdbsum/1ugl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ugl ProSAT], [https://www.topsan.org/Proteins/RSGI/1ugl TOPSAN]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9SE17_BRACM Q9SE17_BRACM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor. | Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor. | ||
| - | + | Structure of the male determinant factor for Brassica self-incompatibility.,Mishima M, Takayama S, Sasaki K, Jee JG, Kojima C, Isogai A, Shirakawa M J Biol Chem. 2003 Sep 19;278(38):36389-95. Epub 2003 Jun 30. PMID:12835321<ref>PMID:12835321</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1ugl" style="background-color:#fffaf0;"></div> |
| - | [[Category: Isogai | + | == References == |
| - | [[Category: Jee | + | <references/> |
| - | [[Category: Kojima | + | __TOC__ |
| - | [[Category: Mishima | + | </StructureSection> |
| - | + | [[Category: Brassica rapa]] | |
| - | [[Category: Sasaki | + | [[Category: Large Structures]] |
| - | [[Category: Shirakawa | + | [[Category: Isogai A]] |
| - | [[Category: Takayama | + | [[Category: Jee JG]] |
| - | + | [[Category: Kojima C]] | |
| - | + | [[Category: Mishima M]] | |
| - | + | [[Category: Sasaki K]] | |
| - | + | [[Category: Shirakawa M]] | |
| - | + | [[Category: Takayama S]] | |
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Current revision
Solution structure of S8-SP11
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Categories: Brassica rapa | Large Structures | Isogai A | Jee JG | Kojima C | Mishima M | Sasaki K | Shirakawa M | Takayama S
