1ule

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[[Image:1ule.jpg|left|200px]]<br /><applet load="1ule" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ule, resolution 2.15&Aring;" />
 
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'''CGL2 in complex with linear B2 trisaccharide'''<br />
 
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==Overview==
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==CGL2 in complex with linear B2 trisaccharide==
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Recognition of and discrimination between potential glyco-substrates is central to the function of galectins. Here we dissect the fundamental parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes reveal that this prototype galectin achieves increased substrate specificity by accommodating substituted oligosaccharides of the mammalian blood group A/B type in an extended binding cleft. Kinetic studies on wild-type and mutant CGL2 proteins demonstrate that the tetrameric organization is essential for functionality. The geometric constraints due to the orthogonal orientation of the four binding sites have important consequences on substrate binding and selectivity.
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<StructureSection load='1ule' size='340' side='right'caption='[[1ule]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ule]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ULE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ULE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ule FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ule OCA], [https://pdbe.org/1ule PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ule RCSB], [https://www.ebi.ac.uk/pdbsum/1ule PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ule ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CGL2_COPCI CGL2_COPCI] Binds lactose. May play a role in fruiting body formation.<ref>PMID:8999822</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ul/1ule_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ule ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1ULE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ULE OCA].
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*[[Galectin 3D structures|Galectin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure and functional analysis of the fungal galectin CGL2., Walser PJ, Haebel PW, Kunzler M, Sargent D, Kues U, Aebi M, Ban N, Structure. 2004 Apr;12(4):689-702. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15062091 15062091]
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__TOC__
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</StructureSection>
[[Category: Coprinopsis cinerea]]
[[Category: Coprinopsis cinerea]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aebi, M.]]
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[[Category: Aebi M]]
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[[Category: Ban, N.]]
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[[Category: Ban N]]
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[[Category: Haebel, P W.]]
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[[Category: Haebel PW]]
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[[Category: Kuenzler, M.]]
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[[Category: Kuenzler M]]
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[[Category: Kues, U.]]
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[[Category: Kues U]]
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[[Category: Walser, P J.]]
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[[Category: Walser PJ]]
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[[Category: beta-galactoside binding lectin]]
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[[Category: galectin]]
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[[Category: lectin]]
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[[Category: sugar binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:47 2008''
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Current revision

CGL2 in complex with linear B2 trisaccharide

PDB ID 1ule

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