This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1gow

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:25, 7 February 2024) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1gow.gif|left|200px]]<br />
 
-
<applet load="1gow" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1gow, resolution 2.6&Aring;" />
 
-
'''BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS'''<br />
 
-
==Overview==
+
==BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS==
-
Enzymes from hyperthermophilic organisms must operate at temperatures, which rapidly denature proteins from mesophiles. The structural basis of, this thermostability is still poorly understood. Towards a further, understanding of hyperthermostability, we have determined the crystal, structure of the beta-glycosidase (clan GH-1A, family 1) from the, hyperthermophilic archaeon Sulfolobus solfataricus at 2.6 A resolution., The enzyme is a tetramer with subunit molecular mass at 60 kDa, and, crystallises with half of the tetramer in the asymmetric unit. The, structure is a (betaalpha)8 barrel, but with substantial elaborations, between the beta-strands and alpha-helices in each repeat. The active site, occurs at the centre of the top face of the barrel and is connected to the, surface by a ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9299327 (full description)]]
+
<StructureSection load='1gow' size='340' side='right'caption='[[1gow]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1gow]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GOW FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gow OCA], [https://pdbe.org/1gow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gow RCSB], [https://www.ebi.ac.uk/pdbsum/1gow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gow ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/BGAL_SACS2 BGAL_SACS2]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/1gow_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gow ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1GOW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]]. Active as [[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23]]. Structure known Active Sites: CTA and CTB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GOW OCA]].
+
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability., Aguilar CF, Sanderson I, Moracci M, Ciaramella M, Nucci R, Rossi M, Pearl LH, J Mol Biol. 1997 Sep 5;271(5):789-802. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9299327 9299327]
+
[[Category: Large Structures]]
-
[[Category: Beta-galactosidase]]
+
[[Category: Saccharolobus solfataricus]]
-
[[Category: Single protein]]
+
[[Category: Aguilar CF]]
-
[[Category: Sulfolobus solfataricus]]
+
[[Category: Pearl LH]]
-
[[Category: Aguilar, C.F.]]
+
[[Category: Sanderson I]]
-
[[Category: Pearl, L.H.]]
+
-
[[Category: Sanderson, I.]]
+
-
[[Category: beta-glycosidase]]
+
-
[[Category: hydrolase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:16:24 2007''
+

Current revision

BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS

PDB ID 1gow

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gow"
Personal tools