1yf2
From Proteopedia
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| - | [[Image:1yf2.png|left|200px]] | ||
| - | + | ==Three-dimensional structure of DNA sequence specificity (S) subunit of a type I restriction-modification enzyme and its functional implications== | |
| - | + | <StructureSection load='1yf2' size='340' side='right'caption='[[1yf2]], [[Resolution|resolution]] 2.40Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1yf2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii_DSM_2661 Methanocaldococcus jannaschii DSM 2661]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YF2 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yf2 OCA], [https://pdbe.org/1yf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yf2 RCSB], [https://www.ebi.ac.uk/pdbsum/1yf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yf2 ProSAT], [https://www.topsan.org/Proteins/BSGC/1yf2 TOPSAN]</span></td></tr> | |
| - | == | + | </table> |
| - | [[1yf2]] is a 2 chain structure with sequence from [ | + | == Function == |
| - | + | [https://www.uniprot.org/uniprot/T1S1_METJA T1S1_METJA] The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity (By similarity). | |
| - | == | + | == Evolutionary Conservation == |
| - | < | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | [ | + | Check<jmol> |
| - | [ | + | <jmolCheckbox> |
| - | [ | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yf/1yf2_consurf.spt"</scriptWhenChecked> |
| - | [ | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | [ | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | [ | + | </jmolCheckbox> |
| - | [[ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yf2 ConSurf]. |
| - | [ | + | <div style="clear:both"></div> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Methanocaldococcus jannaschii DSM 2661]] |
| - | [[Category: | + | [[Category: Adams PD]] |
| - | [[Category: | + | [[Category: Degiovanni A]] |
| - | [[Category: | + | [[Category: Jancarik J]] |
| - | [[Category: | + | [[Category: Kim JS]] |
| - | [[Category: | + | [[Category: Kim R]] |
| + | [[Category: Kim SH]] | ||
| + | [[Category: Yokota HA]] | ||
Current revision
Three-dimensional structure of DNA sequence specificity (S) subunit of a type I restriction-modification enzyme and its functional implications
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