2d45

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[[Image:2d45.png|left|200px]]
 
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{{STRUCTURE_2d45| PDB=2d45 | SCENE= }}
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==Crystal structure of the MecI-mecA repressor-operator complex==
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<StructureSection load='2d45' size='340' side='right'caption='[[2d45]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2d45]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D45 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d45 OCA], [https://pdbe.org/2d45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d45 RCSB], [https://www.ebi.ac.uk/pdbsum/2d45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d45 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MECI_STAAN MECI_STAAN] Transcriptional repressor that constitutively blocks the transcription of the gene for the penicillin-binding protein MecA. Binds palindromic DNA with the sequence 5'-TACA-[AT]-N-TGTA-3'. Regulates genes involved in antibiotic resistance. Binds DNA as a dimer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d4/2d45_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d45 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The dimeric repressor MecI regulates the mecA gene that encodes the penicillin-binding protein PBP-2a in methicillin-resistant Staphylococcus aureus (MRSA). MecI is similar to BlaI, the repressor for the blaZ gene of beta-lactamase. MecI and BlaI can bind to both operator DNA sequences. The crystal structure of MecI in complex with the 32 base-pair cognate DNA of mec was determined to 3.8 A resolution. MecI is a homodimer and each monomer consists of a compact N-terminal winged-helix domain, which binds to DNA, and a loosely packed C-terminal helical domain, which intertwines with its counter-monomer. The crystal contains horizontal layers of virtual DNA double helices extending in three directions, which are separated by perpendicular DNA segments. Each DNA segment is bound to two MecI dimers. Similar to the BlaI-mec complex, but unlike the MecI-bla complex, the MecI repressors bind to both sides of the mec DNA dyad that contains four conserved sequences of TACA/TGTA. The results confirm the up-and-down binding to the mec operator, which may account for cooperative effect of the repressor.
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===Crystal structure of the MecI-mecA repressor-operator complex===
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Structure of the MecI repressor from Staphylococcus aureus in complex with the cognate DNA operator of mec.,Safo MK, Ko TP, Musayev FN, Zhao Q, Wang AH, Archer GL Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Apr 1;62(Pt, 4):320-4. Epub 2006 Mar 25. PMID:16582476<ref>PMID:16582476</ref>
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{{ABSTRACT_PUBMED_16582476}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2d45" style="background-color:#fffaf0;"></div>
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[[2d45]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D45 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:016582476</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Archer, G L.]]
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[[Category: Archer GL]]
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[[Category: Ko, T P.]]
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[[Category: Ko T-P]]
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[[Category: Musayev, F N.]]
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[[Category: Musayev FN]]
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[[Category: Safo, M K.]]
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[[Category: Safo MK]]
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[[Category: Wang, A H.J.]]
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[[Category: Wang AH-J]]
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[[Category: Zhao, Q.]]
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[[Category: Zhao Q]]
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[[Category: B-lactam]]
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[[Category: Dimerization helical domain]]
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[[Category: Methicillin]]
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[[Category: Oligonucleotide binding domain]]
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[[Category: Protein-dna complex]]
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[[Category: Pseudo-dyad dna]]
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[[Category: Repressor]]
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[[Category: Transcription-dna complex]]
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Current revision

Crystal structure of the MecI-mecA repressor-operator complex

PDB ID 2d45

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