2bjq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2bjq" [edit=sysop:move=sysop])
Current revision (09:16, 9 May 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2bjq.png|left|200px]]
 
-
{{STRUCTURE_2bjq| PDB=2bjq | SCENE= }}
+
==Crystal structure of the nematode sperm cell motility protein MFP2==
 +
<StructureSection load='2bjq' size='340' side='right'caption='[[2bjq]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2bjq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJQ FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjq OCA], [https://pdbe.org/2bjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bjq RCSB], [https://www.ebi.ac.uk/pdbsum/2bjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjq ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q7YXK2_ASCSU Q7YXK2_ASCSU]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjq_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bjq ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The simplicity and specialization of the cell motility machinery of Ascaris sperm provides a powerful system in which to probe the basic molecular mechanism of amoeboid cell motility. Although Ascaris sperm locomotion closely resembles that seen in many other types of crawling cell, movement is generated by modulation of a cytoskeleton based on the major sperm protein (MSP) rather than the actin present in other cell types. The Ascaris motility machinery can be studied conveniently in a cell-free in vitro system based on the movement of plasma membrane vesicles by fibres constructed from bundles of MSP filaments. In addition to ATP, MSP and a plasma membrane protein, reconstitution of MSP motility in this cell-free extract requires cytosolic proteins to orchestrate the site-specific assembly and bundling of MSP filaments that generates locomotion. One of these proteins, MFP2, accelerates the rate of movement in this assay. Here, we describe crystal structures of two isoforms of MFP2 and show that both are constructed from two domains that have the same fold based on a novel, compact beta sheet arrangement. Patterns of conservation observed in a structure-based analysis of MFP2 sequences from different nematode species identified regions that may be putative functional interfaces involved both in interactions between MFP2 domains and also with other components of the sperm motility machinery. Analysis of the growth of fibres in vitro in the presence of added MFP2 indicated that MFP2 increases the rate of locomotion by enhancing the effective rate of MSP filament polymerization. This observation, together with the structural data, suggests that MFP2 may function in a manner analogous to formins in actin-based motility.
-
===CRYSTAL STRUCTURE OF THE NEMATODE SPERM CELL MOTILITY PROTEIN MFP2===
+
Structure of MFP2 and its function in enhancing MSP polymerization in Ascaris sperm amoeboid motility.,Grant RP, Buttery SM, Ekman GC, Roberts TM, Stewart M J Mol Biol. 2005 Apr 1;347(3):583-95. PMID:15755452<ref>PMID:15755452</ref>
-
{{ABSTRACT_PUBMED_15755452}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2bjq" style="background-color:#fffaf0;"></div>
-
[[2bjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ascaris_suum Ascaris suum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJQ OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:015755452</ref><references group="xtra"/>
+
</StructureSection>
[[Category: Ascaris suum]]
[[Category: Ascaris suum]]
-
[[Category: Buttery, S M.]]
+
[[Category: Large Structures]]
-
[[Category: Ekman, G C.]]
+
[[Category: Buttery SM]]
-
[[Category: Grant, R P.]]
+
[[Category: Ekman GC]]
-
[[Category: Roberts, T M.]]
+
[[Category: Grant RP]]
-
[[Category: Stewart, M.]]
+
[[Category: Roberts TM]]
-
[[Category: Ascari]]
+
[[Category: Stewart M]]
-
[[Category: Motility]]
+
-
[[Category: Msp]]
+
-
[[Category: Nematode]]
+

Current revision

Crystal structure of the nematode sperm cell motility protein MFP2

PDB ID 2bjq

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bjq"
Personal tools