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1gr7

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[[Image:1gr7.gif|left|200px]]<br />
 
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<applet load="1gr7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gr7, resolution 1.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE DOUBLE MUTANT CYS3SER/SER100PRO FROM PSEUDOMONAS AERUGINOSA AT 1.8 A RESOLUTION'''<br />
 
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==Overview==
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==Crystal structure of the double mutant Cys3Ser/Ser100Pro from Pseudomonas Aeruginosa at 1.8 A resolution==
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Azurin is a cupredoxin, which functions as an electron carrier. Its fold, is dominated by a beta-sheet structure. In the present study, azurin, serves as a model system to investigate the importance of a conserved, disulphide bond for protein stability and folding/unfolding. For this, purpose, we have examined two azurin mutants, the single mutant Cys3Ser, which disrupts azurin's conserved disulphide bond, and the double mutant, Cys3Ser/Ser100Pro, which contains an additional mutation at a site distant, from the conserved disulphide. The crystal structure of the azurin double, mutant has been determined to 1.8 A resolution(2), with a crystallographic, R-factor of 17.5% (R(free)=20.8%). A comparison with the wild-type, structure reveals that structural differences are limited to the sites ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12007613 (full description)]]
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<StructureSection load='1gr7' size='340' side='right'caption='[[1gr7]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [https://pdbe.org/1gr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gr7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/1gr7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gr7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GR7 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: CUD. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]].
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*[[Azurin 3D structures|Azurin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the double azurin mutant Cys3Ser/Ser100Pro from Pseudomonas aeruginosa at 1.8 A resolution: its folding-unfolding energy and unfolding kinetics., Okvist M, Bonander N, Sandberg A, Karlsson BG, Krengel U, Xue Y, Sjolin L, Biochim Biophys Acta. 2002 Apr 29;1596(2):336-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12007613 12007613]
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Bonander N]]
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[[Category: Bonander, N.]]
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[[Category: Karlsson BG]]
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[[Category: Karlsson, B.G.]]
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[[Category: Krengel U]]
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[[Category: Krengel, U.]]
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[[Category: Okvist M]]
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[[Category: Okvist, M.]]
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[[Category: Sandberg A]]
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[[Category: Sandberg, A.]]
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[[Category: Sjolin L]]
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[[Category: Sjolin, L.]]
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[[Category: Xue Y]]
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[[Category: Xue, Y.]]
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[[Category: CU]]
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[[Category: electron transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:17:50 2007''
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Current revision

Crystal structure of the double mutant Cys3Ser/Ser100Pro from Pseudomonas Aeruginosa at 1.8 A resolution

PDB ID 1gr7

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