This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.


Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1uyq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:23, 9 May 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1uyq.gif|left|200px]]<br /><applet load="1uyq" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1uyq, resolution 2.2&Aring;" />
 
-
'''MUTATED B-GLUCOSIDASE A FROM PAENIBACILLUS POLYMYXA SHOWING INCREASED STABILITY'''<br />
 
-
==About this Structure==
+
==mutated b-glucosidase A from Paenibacillus polymyxa showing increased stability==
-
1UYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paenibacillus_polymyxa Paenibacillus polymyxa] with <scene name='pdbligand=G2F:'>G2F</scene> and <scene name='pdbligand=NFG:'>NFG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] Known structural/functional Site: <scene name='pdbsite=CAT:Nfg+Binding+Site+For+Chain+A'>CAT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UYQ OCA].
+
<StructureSection load='1uyq' size='340' side='right'caption='[[1uyq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
[[Category: Beta-glucosidase]]
+
== Structural highlights ==
-
[[Category: Paenibacillus polymyxa]]
+
<table><tr><td colspan='2'>[[1uyq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_polymyxa Paenibacillus polymyxa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UYQ FirstGlance]. <br>
-
[[Category: Single protein]]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
[[Category: Isorna, P.]]
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=NFG:2,4-DINITROPHENYL+2-DEOXY-2-FLUORO-BETA-D-GLUCOPYRANOSIDE'>NFG</scene></td></tr>
-
[[Category: Polaina, J.]]
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uyq OCA], [https://pdbe.org/1uyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uyq RCSB], [https://www.ebi.ac.uk/pdbsum/1uyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uyq ProSAT]</span></td></tr>
-
[[Category: Sanz-Aparicio, J.]]
+
</table>
-
[[Category: G2F]]
+
== Function ==
-
[[Category: NFG]]
+
[https://www.uniprot.org/uniprot/BGLA_PAEPO BGLA_PAEPO] BglA is intracellular and cleaves cellobiose probably through inorganic phosphate mediated hydrolysis.
-
[[Category: b-glucosidase]]
+
== Evolutionary Conservation ==
-
[[Category: cellulose degradation]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: hydrolase glycosidase]]
+
Check<jmol>
-
[[Category: mutation affecting stability]]
+
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uy/1uyq_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uyq ConSurf].
 +
<div style="clear:both"></div>
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:29:46 2008''
+
==See Also==
 +
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]]
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Paenibacillus polymyxa]]
 +
[[Category: Isorna P]]
 +
[[Category: Polaina J]]
 +
[[Category: Sanz-Aparicio J]]

Current revision

mutated b-glucosidase A from Paenibacillus polymyxa showing increased stability

PDB ID 1uyq

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools