2chq

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[[Image:2chq.png|left|200px]]
 
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{{STRUCTURE_2chq| PDB=2chq | SCENE= }}
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==Replication Factor C ADPNP complex==
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<StructureSection load='2chq' size='340' side='right'caption='[[2chq]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2chq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CHQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2chq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2chq OCA], [https://pdbe.org/2chq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2chq RCSB], [https://www.ebi.ac.uk/pdbsum/2chq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2chq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RFCS_ARCFU RFCS_ARCFU] Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.<ref>PMID:12384579</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/2chq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2chq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex.
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===REPLICATION FACTOR C ADPNP COMPLEX===
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Communication between subunits within an archaeal clamp-loader complex.,Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB EMBO J. 2006 May 17;25(10):2209-18. Epub 2006 Apr 20. PMID:16628222<ref>PMID:16628222</ref>
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{{ABSTRACT_PUBMED_16628222}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2chq" style="background-color:#fffaf0;"></div>
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[[2chq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHQ OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:016628222</ref><references group="xtra"/>
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</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Cook, N.]]
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[[Category: Large Structures]]
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[[Category: Hall, D R.]]
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[[Category: Cook N]]
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[[Category: Seybert, A.]]
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[[Category: Hall DR]]
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[[Category: Singleton, M R.]]
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[[Category: Seybert A]]
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[[Category: Wigley, D B.]]
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[[Category: Singleton MR]]
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[[Category: Aaa+ atpase]]
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[[Category: Wigley DB]]
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[[Category: Atp-binding]]
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[[Category: Clamp loader]]
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[[Category: Dna replication]]
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[[Category: Dna-binding protein]]
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[[Category: Nucleotide-binding]]
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Current revision

Replication Factor C ADPNP complex

PDB ID 2chq

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