2bht

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[[Image:2bht.png|left|200px]]
 
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{{STRUCTURE_2bht| PDB=2bht | SCENE= }}
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==Crystal structure of O-acetylserine sulfhydrylase B==
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<StructureSection load='2bht' size='340' side='right'caption='[[2bht]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bht]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BHT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bht OCA], [https://pdbe.org/2bht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bht RCSB], [https://www.ebi.ac.uk/pdbsum/2bht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bht ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYSM_ECOLI CYSM_ECOLI] Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/2bht_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bht ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzyme O-acetylserine sulfhydrylase participates in the biosynthesis of l-cysteine in bacteria and plants. The structure of isoenzyme B (CysM) from Escherichia coli was established in a hexagonal crystal form at 2.7 A resolution (wild-type) and in a merohedrally twinned tetragonal crystal form at 2.1 A resolution (surface mutant). Structural superpositions revealed the variations with respect to isoenzyme A (CysK) and explained the different substrate specificities. A geometric model of the reaction catalyzed by CysM is proposed. Both isoenzymes are used for the production of l-amino acid derivatives as building blocks for the synthesis of peptides and peptidomimetic drugs. Since the structure of CysM revealed a remarkable main chain variation at the active center, it constitutes a further starting point for engineering mutants with novel substrate specificities.
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===CRYSTAL STRUCTURE OF O-ACETYLSERINE SULFHYDRYLASE B===
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Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli.,Claus MT, Zocher GE, Maier TH, Schulz GE Biochemistry. 2005 Jun 21;44(24):8620-6. PMID:15952768<ref>PMID:15952768</ref>
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{{ABSTRACT_PUBMED_15952768}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2bht" style="background-color:#fffaf0;"></div>
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[[2bht]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHT OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015952768</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Cysteine synthase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Claus, M T.]]
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[[Category: Large Structures]]
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[[Category: Maier, T H.P.]]
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[[Category: Claus MT]]
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[[Category: Schulz, G E.]]
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[[Category: Maier THP]]
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[[Category: Zocher, G E.]]
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[[Category: Schulz GE]]
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[[Category: Cysteine biosynthesis]]
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[[Category: Zocher GE]]
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[[Category: Plp-dependent enzyme]]
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[[Category: Pyridoxal phosphate]]
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[[Category: Pyridoxal-5'-phosphate]]
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[[Category: Transferase]]
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Current revision

Crystal structure of O-acetylserine sulfhydrylase B

PDB ID 2bht

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