2agm

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[[Image:2agm.png|left|200px]]
 
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{{STRUCTURE_2agm| PDB=2agm | SCENE= }}
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==Solution structure of the R-module from AlgE4==
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<StructureSection load='2agm' size='340' side='right'caption='[[2agm]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2agm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AGM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2agm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2agm OCA], [https://pdbe.org/2agm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2agm RCSB], [https://www.ebi.ac.uk/pdbsum/2agm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2agm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALGE4_AZOVI ALGE4_AZOVI] Converts beta-D-mannuronic acid (M) to alpha-L-guluronic acid (G), but introduces almost exclusively MG blocks, producing a polymer with non-gel-forming capacity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ag/2agm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2agm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the bacterium Azotobacter vinelandii, a family of seven secreted and calcium-dependent mannuronan C-5 epimerases (AlgE1-7) has been identified. These epimerases are responsible for the epimerization of beta-d-mannuronic acid to alpha-l-guluronic acid in alginate polymers. The epimerases consist of two types of structural modules, designated A (one or two copies) and R (one to seven copies). The structure of the catalytically active A-module from the smallest epimerase AlgE4 (consisting of AR) has been solved recently. This paper describes the NMR structure of the R-module from AlgE4 and its titration with a substrate analogue and paramagnetic thulium ions. The R-module folds into a right-handed parallel beta-roll. The overall shape of the R-module is an elongated molecule with a positively charged patch that interacts with the substrate. Titration of the R-module with thulium indicated possible calcium binding sites in the loops formed by the nonarepeat sequences in the N-terminal part of the molecule and the importance of calcium binding for the stability of the R-module. Structure calculations showed that calcium ions can be incorporated in these loops without structural violations and changes. Based on the structure and the electrostatic surface potential of both the A- and R-module from AlgE4, a model for the appearance of the whole protein is proposed.
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===Solution structure of the R-module from AlgE4===
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NMR structure of the R-module: a parallel beta-roll subunit from an Azotobacter vinelandii mannuronan C-5 epimerase.,Aachmann FL, Svanem BI, Guntert P, Petersen SB, Valla S, Wimmer R J Biol Chem. 2006 Mar 17;281(11):7350-6. Epub 2006 Jan 3. PMID:16407237<ref>PMID:16407237</ref>
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{{ABSTRACT_PUBMED_16407237}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2agm" style="background-color:#fffaf0;"></div>
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[[2agm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AGM OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:016407237</ref><references group="xtra"/>
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</StructureSection>
[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
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[[Category: Aachmann, F L.]]
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[[Category: Large Structures]]
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[[Category: Guntert, P.]]
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[[Category: Aachmann FL]]
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[[Category: Petersen, S B.]]
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[[Category: Guntert P]]
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[[Category: Svanem, B I.]]
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[[Category: Petersen SB]]
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[[Category: Valla, S.]]
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[[Category: Svanem BI]]
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[[Category: Wimmer, R.]]
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[[Category: Valla S]]
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[[Category: Isomerase]]
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[[Category: Wimmer R]]
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[[Category: Parallel beta-roll]]
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Current revision

Solution structure of the R-module from AlgE4

PDB ID 2agm

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