2pjw

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[[Image:2pjw.png|left|200px]]
 
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{{STRUCTURE_2pjw| PDB=2pjw | SCENE= }}
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==The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting==
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<StructureSection load='2pjw' size='340' side='right'caption='[[2pjw]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pjw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PJW FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1yd8|1yd8]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hse1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), VPS27, GRD11 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pjw OCA], [https://pdbe.org/2pjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pjw RCSB], [https://www.ebi.ac.uk/pdbsum/2pjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pjw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/HSE1_YEAST HSE1_YEAST]] Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.<ref>PMID:12055639</ref> <ref>PMID:14581452</ref> <ref>PMID:15086794</ref> <ref>PMID:17079730</ref> [[https://www.uniprot.org/uniprot/VPS27_YEAST VPS27_YEAST]] Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane. Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi. Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC. Might also function as an alternate adapter in the COPIb clathrin-like coat.<ref>PMID:3062374</ref> <ref>PMID:1493335</ref> <ref>PMID:8649377</ref> <ref>PMID:9015300</ref> <ref>PMID:9265642</ref> <ref>PMID:11208109</ref> <ref>PMID:11416128</ref> <ref>PMID:12055639</ref> <ref>PMID:11872141</ref> <ref>PMID:12900393</ref> <ref>PMID:14581452</ref> <ref>PMID:15166140</ref> <ref>PMID:15107463</ref> <ref>PMID:15086794</ref> <ref>PMID:17101773</ref> <ref>PMID:17135292</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pj/2pjw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pjw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The yeast Vps27/Hse1 complex and the homologous mammalian Hrs/STAM complex deliver ubiquitinated transmembrane proteins to the ESCRT endosomal-sorting pathway. The Vps27/Hse1 complex directly binds to ubiquitinated transmembrane proteins and recruits both ubiquitin ligases and deubiquitinating enzymes. We have solved the crystal structure of the core responsible for the assembly of the Vps27/Hse1 complex at 3.0 A resolution. The structure consists of two intertwined GAT domains, each consisting of two helices from one subunit and one from the other. The two GAT domains are connected by an antiparallel coiled coil, forming a 90 A-long barbell-like structure. This structure places the domains of Vps27 and Hse1 that recruit ubiquitinated cargo and deubiquitinating enzymes close to each other. Coarse-grained Monte Carlo simulations of the Vps27/Hse1 complex on a membrane show how the complex binds cooperatively to lipids and ubiquitinated membrane proteins and acts as a scaffold for ubiquitination reactions.
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===The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting===
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The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting.,Prag G, Watson H, Kim YC, Beach BM, Ghirlando R, Hummer G, Bonifacino JS, Hurley JH Dev Cell. 2007 Jun;12(6):973-86. PMID:17543868<ref>PMID:17543868</ref>
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{{ABSTRACT_PUBMED_17543868}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2pjw" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2pjw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJW OCA].
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*[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017543868</ref><references group="xtra"/>
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__TOC__
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[[Category: Saccharomyces cerevisiae]]
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</StructureSection>
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[[Category: Hurley, J H.]]
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[[Category: Atcc 18824]]
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[[Category: Prag, G.]]
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[[Category: Large Structures]]
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[[Category: Hurley, J H]]
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[[Category: Prag, G]]
[[Category: Core complex]]
[[Category: Core complex]]
[[Category: Doamin swap]]
[[Category: Doamin swap]]
[[Category: Endocytosis-exocytosis complex]]
[[Category: Endocytosis-exocytosis complex]]
[[Category: Gat domain]]
[[Category: Gat domain]]

Current revision

The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting

PDB ID 2pjw

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