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2nu5

From Proteopedia

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[[Image:2nu5.png|left|200px]]
 
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{{STRUCTURE_2nu5| PDB=2nu5 | SCENE= }}
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==Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine==
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<StructureSection load='2nu5' size='340' side='right'caption='[[2nu5]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nu5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Griffithsia_sp._Q66D336 Griffithsia sp. Q66D336]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NU5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.564&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nu5 OCA], [https://pdbe.org/2nu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nu5 RCSB], [https://www.ebi.ac.uk/pdbsum/2nu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nu5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GRFIN_GRISQ GRFIN_GRISQ]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/2nu5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nu5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.
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===Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine===
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Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine.,Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736<ref>PMID:17567736</ref>
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{{ABSTRACT_PUBMED_17567736}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2nu5" style="background-color:#fffaf0;"></div>
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[[2nu5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Griffithsia_sp._q66d336 Griffithsia sp. q66d336]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NU5 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:017567736</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Griffithsia sp. q66d336]]
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[[Category: Griffithsia sp. Q66D336]]
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[[Category: Wlodawer, A.]]
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[[Category: Large Structures]]
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[[Category: Ziolkowska, N E.]]
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[[Category: Wlodawer A]]
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[[Category: Antiviral protein]]
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[[Category: Ziolkowska NE]]
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[[Category: Domain swapping]]
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[[Category: Griffithsin]]
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[[Category: Hiv]]
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[[Category: Lectin]]
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[[Category: Mannose binding]]
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[[Category: Sar]]
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[[Category: Sugar binding protein]]
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Current revision

Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine

PDB ID 2nu5

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