2nu5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2nu5" [edit=sysop:move=sysop])
Current revision (08:21, 30 October 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2nu5.png|left|200px]]
 
-
{{STRUCTURE_2nu5| PDB=2nu5 | SCENE= }}
+
==Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine==
 +
<StructureSection load='2nu5' size='340' side='right'caption='[[2nu5]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2nu5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Griffithsia_sp._Q66D336 Griffithsia sp. Q66D336]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NU5 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.564&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nu5 OCA], [https://pdbe.org/2nu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nu5 RCSB], [https://www.ebi.ac.uk/pdbsum/2nu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nu5 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GRFIN_GRISQ GRFIN_GRISQ]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nu/2nu5_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nu5 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.
-
===Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine===
+
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine.,Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736<ref>PMID:17567736</ref>
-
{{ABSTRACT_PUBMED_17567736}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2nu5" style="background-color:#fffaf0;"></div>
-
[[2nu5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Griffithsia_sp._q66d336 Griffithsia sp. q66d336]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NU5 OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:017567736</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Griffithsia sp. q66d336]]
+
[[Category: Griffithsia sp. Q66D336]]
-
[[Category: Wlodawer, A.]]
+
[[Category: Large Structures]]
-
[[Category: Ziolkowska, N E.]]
+
[[Category: Wlodawer A]]
-
[[Category: Antiviral protein]]
+
[[Category: Ziolkowska NE]]
-
[[Category: Domain swapping]]
+
-
[[Category: Griffithsin]]
+
-
[[Category: Hiv]]
+
-
[[Category: Lectin]]
+
-
[[Category: Mannose binding]]
+
-
[[Category: Sar]]
+
-
[[Category: Sugar binding protein]]
+

Current revision

Crystal structure of a complex of griffithsin cocrystallized with N-acetylglucosamine

PDB ID 2nu5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2nu5"
Personal tools