2ge4

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[[Image:2ge4.png|left|200px]]
 
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{{STRUCTURE_2ge4| PDB=2ge4 | SCENE= }}
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==High-resolution solution structure of outer membrane protein A transmembrane domain==
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<StructureSection load='2ge4' size='340' side='right'caption='[[2ge4]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ge4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GE4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ge4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ge4 OCA], [https://pdbe.org/2ge4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ge4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ge4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ge4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OMPA_ECOLI OMPA_ECOLI] Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2ge4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ge4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure determination of membrane proteins is one of the most challenging applications of solution NMR spectroscopy. The paucity of distance information available from the highly deuterated proteins employed requires new approaches in structure determination. Here we demonstrate that significant improvement in the structure accuracy of the membrane protein OmpA can be achieved by refinement with residual dipolar couplings (RDCs). The application of charged polyacrylamide gels allowed us to obtain two alignments and accurately measure numerous heteronuclear dipolar couplings. Furthermore, we have demonstrated that using a large set of RDCs in the refinement can yield a structure with 1 A rms deviation to the backbone of the high-resolution crystal structure. Our simulations with various data sets indicate that dipolar couplings will be critical for obtaining accurate structures of membrane proteins.
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===High-resolution solution structure of outer membrane protein A transmembrane domain===
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Increasing the accuracy of solution NMR structures of membrane proteins by application of residual dipolar couplings. High-resolution structure of outer membrane protein A.,Cierpicki T, Liang B, Tamm LK, Bushweller JH J Am Chem Soc. 2006 May 31;128(21):6947-51. PMID:16719475<ref>PMID:16719475</ref>
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{{ABSTRACT_PUBMED_16719475}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ge4" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2ge4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GE4 OCA].
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*[[Porin 3D structures|Porin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016719475</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Bushweller, J H.]]
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[[Category: Large Structures]]
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[[Category: Cierpicki, T.]]
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[[Category: Bushweller JH]]
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[[Category: Liang, B.]]
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[[Category: Cierpicki T]]
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[[Category: Tamm, L K.]]
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[[Category: Liang B]]
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[[Category: Beta barrel]]
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[[Category: Tamm LK]]
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[[Category: Membrane protein]]
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Current revision

High-resolution solution structure of outer membrane protein A transmembrane domain

PDB ID 2ge4

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