1w7z
From Proteopedia
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| - | [[Image:1w7z.gif|left|200px]]<br /><applet load="1w7z" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1w7z, resolution 1.67Å" /> | ||
| - | '''CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)'''<br /> | ||
| - | == | + | ==Crystal structure of the free (uncomplexed) Ecballium elaterium trypsin inhibitor (EETI-II)== |
| + | <StructureSection load='1w7z' size='340' side='right'caption='[[1w7z]], [[Resolution|resolution]] 1.67Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1w7z]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W7Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w7z OCA], [https://pdbe.org/1w7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w7z RCSB], [https://www.ebi.ac.uk/pdbsum/1w7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w7z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ITR2_ECBEL ITR2_ECBEL] Inhibits trypsin. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold. | The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold. | ||
| - | + | Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold.,Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759<ref>PMID:16131759</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1w7z" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Ecballium elaterium]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Debreczeni JE]] | ||
| + | [[Category: Kolmar H]] | ||
| + | [[Category: Kraetzner R]] | ||
| + | [[Category: Pape T]] | ||
| + | [[Category: Scheldrick GM]] | ||
| + | [[Category: Schneider TR]] | ||
| + | [[Category: Uson I]] | ||
Current revision
Crystal structure of the free (uncomplexed) Ecballium elaterium trypsin inhibitor (EETI-II)
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