1wae
From Proteopedia
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| - | [[Image:1wae.gif|left|200px]]<br /><applet load="1wae" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1wae, resolution 1.95Å" /> | ||
| - | '''CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE'''<br /> | ||
| - | == | + | ==Crystal structure of H129V Mutant of Alcaligenes Xylosoxidans Nitrite Reductase== |
| + | <StructureSection load='1wae' size='340' side='right'caption='[[1wae]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1wae]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAE FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wae OCA], [https://pdbe.org/1wae PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wae RCSB], [https://www.ebi.ac.uk/pdbsum/1wae PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wae ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O68601_ALCXX O68601_ALCXX] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wae_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wae ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
Copper nitrite reductases contain both an electron-transfer type 1 Cu site and a catalytic type 2 Cu site. We have mutated one of the type 2 copper ligating histidines to observe the effect on catalytic turnover. This mutation has created a unique site where Cu is ligated by 2 His Nepsilon2 atoms alone. | Copper nitrite reductases contain both an electron-transfer type 1 Cu site and a catalytic type 2 Cu site. We have mutated one of the type 2 copper ligating histidines to observe the effect on catalytic turnover. This mutation has created a unique site where Cu is ligated by 2 His Nepsilon2 atoms alone. | ||
| - | + | Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase.,Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS Inorg Chem. 2004 Nov 29;43(24):7591-3. PMID:15554622<ref>PMID:15554622</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1wae" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Achromobacter xylosoxidans]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Antonyuk SV]] | ||
| + | [[Category: Eady RR]] | ||
| + | [[Category: Ellis MJ]] | ||
| + | [[Category: Hasnain SS]] | ||
| + | [[Category: Sawers G]] | ||
| + | [[Category: Strange RW]] | ||
Current revision
Crystal structure of H129V Mutant of Alcaligenes Xylosoxidans Nitrite Reductase
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