2jja
From Proteopedia
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| - | [[Image:2jja.png|left|200px]] | ||
| - | + | ==Crystal structure of GNA with synthetic copper base pair== | |
| + | <StructureSection load='2jja' size='340' side='right'caption='[[2jja]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2jja]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JJA FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene>, <scene name='pdbligand=ZAD:(S)-1-(2,3-DIHYDROXYPROPYL)-ADENINE'>ZAD</scene>, <scene name='pdbligand=ZCY:(S)-1-(2,3-DIHYDROXYPROPYL)-CYTOSINE'>ZCY</scene>, <scene name='pdbligand=ZGU:(S)-1-(2,3-DIHYDROXYPROPYL)-GUANINE'>ZGU</scene>, <scene name='pdbligand=ZHP:(S)-1-(2,3-DIHYDROXYPROPYL)-HYDROXYPYRIDONE'>ZHP</scene>, <scene name='pdbligand=ZTH:(S)-1-(2,3-DIHYDROXYPROPYL)-THYMINE'>ZTH</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jja OCA], [https://pdbe.org/2jja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jja RCSB], [https://www.ebi.ac.uk/pdbsum/2jja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jja ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of an 8-mer (S)-GNA duplex is presented. As a tool for phasing, the anomalous diffraction of two copper(II) ions within two artificial metallo-base pairs was employed. The duplex structure confirms a canonical Watson-Crick base pairing scheme of GNA with antiparallel strands. The duplex secondary structure is distinct from canonical A- and B-form nucleic acids and can be described as a right-handed helical ribbon wrapped around the helix axis, resulting in a large hollow core. Most intriguingly, neighboring base pairs slide strongly against each other, resulting in extensive interstrand base-base hydrophobic interactions along with unusual hydrophobic intrastrand interactions of nucleobases with their backbone. These results reveal how a minimal nucleic acid backbone can support highly stable Watson-Crick-like duplex formation. | ||
| - | + | Duplex structure of a minimal nucleic acid.,Schlegel MK, Essen LO, Meggers E J Am Chem Soc. 2008 Jul 2;130(26):8158-9. Epub 2008 Jun 5. PMID:18529005<ref>PMID:18529005</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2jja" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | < | + | </StructureSection> |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Synthetic construct]] | |
| - | [[Category: | + | [[Category: Essen L-O]] |
| - | [[Category: | + | [[Category: Meggers E]] |
| - | [[Category: | + | [[Category: Schlegel MK]] |
| - | [[Category: | + | |
| - | [[Category: | + | |
| - | + | ||
Current revision
Crystal structure of GNA with synthetic copper base pair
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