1gz9

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[[Image:1gz9.gif|left|200px]]<br />
 
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<applet load="1gz9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gz9, resolution 1.70&Aring;" />
 
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'''HIGH-RESOLUTION CRYSTAL STRUCTURE OF ERYTHRINA CRISTAGALLI LECTIN IN COMPLEX WITH 2'-ALPHA-L-FUCOSYLLACTOSE'''<br />
 
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==Overview==
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==High-Resolution Crystal Structure of Erythrina cristagalli Lectin in Complex with 2'-alpha-L-Fucosyllactose==
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The primary sequence of Erythrina cristagalli lectin (ECL) was mapped by, mass spectrometry, and the crystal structures of the lectin in complex, with lactose and 2'-alpha-L-fucosyllactose were determined at 1.6A and, 1.7A resolution, respectively. The two complexes were compared with the, crystal structure of the closely related Erythrina corallodendron lectin, (ECorL) in complex with lactose, with the crystal structure of the Ulex, europaeus lectin II in complex with 2'-alpha-L-fucosyllactose, and with, two modeled complexes of ECorL with, 2'-alpha-L-fucosyl-N-acetyllactosamine. The molecular models are very, similar to the crystal structure of ECL in complex with, 2'-alpha-L-fucosyllactose with respect to the overall mode of binding, with the L-fucose fitting snugly into the cavity ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12139934 (full description)]]
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<StructureSection load='1gz9' size='340' side='right'caption='[[1gz9]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gz9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Erythrina_crista-galli Erythrina crista-galli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GZ9 FirstGlance]. <br>
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1GZ9 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Eukaryota Eukaryota]] with MN and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: MNA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZ9 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900070:2-fucosyllactose'>PRD_900070</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gz9 OCA], [https://pdbe.org/1gz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gz9 RCSB], [https://www.ebi.ac.uk/pdbsum/1gz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gz9 ProSAT]</span></td></tr>
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High-resolution crystal structures of Erythrina cristagalli lectin in complex with lactose and 2'-alpha-L-fucosyllactose and correlation with thermodynamic binding data., Svensson C, Teneberg S, Nilsson CL, Kjellberg A, Schwarz FP, Sharon N, Krengel U, J Mol Biol. 2002 Aug 2;321(1):69-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12139934 12139934]
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</table>
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[[Category: Eukaryota]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/LEC_ERYCG LEC_ERYCG]
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[[Category: Kjellberg, A.]]
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== Evolutionary Conservation ==
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[[Category: Krengel, U.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Nilsson, C.L.]]
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Check<jmol>
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[[Category: Schwarz, F.P.]]
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<jmolCheckbox>
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[[Category: Sharon, N.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/1gz9_consurf.spt"</scriptWhenChecked>
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[[Category: Svensson, C.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Teneberg, S.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: CA]]
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</jmolCheckbox>
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[[Category: MN]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gz9 ConSurf].
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[[Category: carbohydrate]]
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<div style="clear:both"></div>
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[[Category: crystal structure]]
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__TOC__
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[[Category: fucose]]
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</StructureSection>
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[[Category: fucosyllactose protein-carbohydrate interactions]]
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[[Category: Erythrina crista-galli]]
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[[Category: glycobiology]]
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[[Category: Large Structures]]
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[[Category: glycoprotein]]
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[[Category: Kjellberg A]]
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[[Category: lactose]]
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[[Category: Krengel U]]
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[[Category: legume lectin]]
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[[Category: Nilsson CL]]
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[[Category: protein]]
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[[Category: Schwarz FP]]
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[[Category: saccharide]]
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[[Category: Sharon N]]
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[[Category: Svensson C]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:23:24 2007''
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[[Category: Teneberg S]]

Current revision

High-Resolution Crystal Structure of Erythrina cristagalli Lectin in Complex with 2'-alpha-L-Fucosyllactose

PDB ID 1gz9

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