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1gza

From Proteopedia

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[[Image:1gza.gif|left|200px]]<br />
 
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<applet load="1gza" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gza, resolution 2.06&Aring;" />
 
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'''PEROXIDASE'''<br />
 
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==Overview==
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==PEROXIDASE==
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The site and characteristics of iodide binding to Arthromyces ramosus, peroxidase were examined by x-ray crystallographic analysis, 1H and 127I, NMR, and kinetic studies. X-ray analysis of an A. ramosus peroxidase, crystal soaked in a KI solution at pH 5.5 showed that a single iodide ion, is located at the entrance of the access channel to the distal side of the, heme and lies between the two peptide segments, Phe90-Pro91-Ala92 and, Ser151-Leu152-Ile153, 12.8 A from the heme iron. The distances between the, iodide ion and heme peripheral methyl groups were all more than 10 A. The, findings agree with the results obtained with 1H NMR in which the chemical, shift and intensity of the methyl groups in the hyperfine shift region of, A. ramosus peroxidase were hardly affected by the addition ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9038188 (full description)]]
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<StructureSection load='1gza' size='340' side='right'caption='[[1gza]], [[Resolution|resolution]] 2.06&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gza]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agaricales_sp._'Arthromyces_ramosus' Agaricales sp. 'Arthromyces ramosus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GZA FirstGlance]. <br>
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1GZA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Eukaryota Eukaryota]] with CA, IOD and HEM as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Peroxidase Peroxidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7]]. Structure known Active Sites: CA1, CA2 and GL1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GZA OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gza OCA], [https://pdbe.org/1gza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gza RCSB], [https://www.ebi.ac.uk/pdbsum/1gza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gza ProSAT]</span></td></tr>
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Binding of iodide to Arthromyces ramosus peroxidase investigated with X-ray crystallographic analysis, 1H and 127I NMR spectroscopy, and steady-state kinetics., Fukuyama K, Sato K, Itakura H, Takahashi S, Hosoya T, J Biol Chem. 1997 Feb 28;272(9):5752-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9038188 9038188]
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</table>
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[[Category: Eukaryota]]
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== Function ==
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[[Category: Peroxidase]]
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[https://www.uniprot.org/uniprot/PER_ARTRA PER_ARTRA]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Fukuyama, K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Itakura, H.]]
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Check<jmol>
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[[Category: CA]]
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<jmolCheckbox>
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[[Category: HEM]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/1gza_consurf.spt"</scriptWhenChecked>
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[[Category: IOD]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: glycoprotein]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: heme]]
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</jmolCheckbox>
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[[Category: oxidoreductase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gza ConSurf].
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[[Category: peroxidase]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:23:26 2007''
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</StructureSection>
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[[Category: Agaricales sp. 'Arthromyces ramosus']]
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[[Category: Large Structures]]
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[[Category: Fukuyama K]]
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[[Category: Itakura H]]

Current revision

PEROXIDASE

PDB ID 1gza

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