2klq

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[[Image:2klq.png|left|200px]]
 
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{{STRUCTURE_2klq| PDB=2klq | SCENE= }}
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==The solution structure of CBD of human MCM6==
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<StructureSection load='2klq' size='340' side='right'caption='[[2klq]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2klq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KLQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KLQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2klq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2klq OCA], [https://pdbe.org/2klq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2klq RCSB], [https://www.ebi.ac.uk/pdbsum/2klq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2klq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCM6_HUMAN MCM6_HUMAN] Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.<ref>PMID:9305914</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/2klq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2klq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The minichromosome maintenance (Mcm) 2-7 complex is the replicative helicase in eukaryotic species, and it plays essential roles in the initiation and elongation phases of DNA replication. During late M and early G(1), the Mcm2-7 complex is loaded onto chromatin to form prereplicative complex in a Cdt1-dependent manner. However, the detailed molecular mechanism of this loading process is still elusive. In this study, we demonstrate that the previously uncharacterized C-terminal domain of human Mcm6 is the Cdt1 binding domain (CBD) and present its high resolution NMR structure. The structure of CBD exhibits a typical "winged helix" fold that is generally involved in protein-nucleic acid interaction. Nevertheless, the CBD failed to interact with DNA in our studies, indicating that it is specific for protein-protein interaction. The CBD-Cdt1 interaction involves the helix-turn-helix motif of CBD. The results reported here provide insight into the molecular mechanism of Mcm2-7 chromatin loading and prereplicative complex assembly.
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===The solution structure of CBD of human MCM6===
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Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6.,Wei Z, Liu C, Wu X, Xu N, Zhou B, Liang C, Zhu G J Biol Chem. 2010 Apr 23;285(17):12469-73. Epub 2010 Mar 4. PMID:20202939<ref>PMID:20202939</ref>
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{{ABSTRACT_PUBMED_20202939}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2klq" style="background-color:#fffaf0;"></div>
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[[2klq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KLQ OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:020202939</ref><references group="xtra"/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Liu, C.]]
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[[Category: Large Structures]]
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[[Category: Atp-binding]]
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[[Category: Liu C]]
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[[Category: Cbd structure]]
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[[Category: Cdt1]]
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[[Category: Cell cycle]]
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[[Category: Dna replication]]
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[[Category: Dna-binding]]
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[[Category: Mcm6]]
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[[Category: Nucleotide-binding]]
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[[Category: Nucleus]]
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[[Category: Phosphoprotein]]
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[[Category: Pre-rc]]
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[[Category: Replication]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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Current revision

The solution structure of CBD of human MCM6

PDB ID 2klq

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