2k7w

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[[Image:2k7w.png|left|200px]]
 
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{{STRUCTURE_2k7w| PDB=2k7w | SCENE= }}
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==BAX Activation is Initiated at a Novel Interaction Site==
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<StructureSection load='2k7w' size='340' side='right'caption='[[2k7w]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2k7w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K7W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7w OCA], [https://pdbe.org/2k7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k7w RCSB], [https://www.ebi.ac.uk/pdbsum/2k7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BAX_HUMAN BAX_HUMAN] Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.<ref>PMID:8358790</ref> <ref>PMID:10772918</ref> <ref>PMID:8521816</ref> <ref>PMID:16113678</ref> <ref>PMID:18948948</ref> <ref>PMID:21199865</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/2k7w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k7w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BAX is a pro-apoptotic protein of the BCL-2 family that is stationed in the cytosol until activated by a diversity of stress stimuli to induce cell death. Anti-apoptotic proteins such as BCL-2 counteract BAX-mediated cell death. Although an interaction site that confers survival functionality has been defined for anti-apoptotic proteins, an activation site has not been identified for BAX, rendering its explicit trigger mechanism unknown. We previously developed stabilized alpha-helix of BCL-2 domains (SAHBs) that directly initiate BAX-mediated mitochondrial apoptosis. Here we demonstrate by NMR analysis that BIM SAHB binds BAX at an interaction site that is distinct from the canonical binding groove characterized for anti-apoptotic proteins. The specificity of the human BIM-SAHB-BAX interaction is highlighted by point mutagenesis that disrupts functional activity, confirming that BAX activation is initiated at this novel structural location. Thus, we have now defined a BAX interaction site for direct activation, establishing a new target for therapeutic modulation of apoptosis.
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===BAX Activation is Initiated at a Novel Interaction Site===
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BAX activation is initiated at a novel interaction site.,Gavathiotis E, Suzuki M, Davis ML, Pitter K, Bird GH, Katz SG, Tu HC, Kim H, Cheng EH, Tjandra N, Walensky LD Nature. 2008 Oct 23;455(7216):1076-81. PMID:18948948<ref>PMID:18948948</ref>
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{{ABSTRACT_PUBMED_18948948}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2k7w" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2k7w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7W OCA].
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*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018948948</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Bird, G H.]]
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[[Category: Large Structures]]
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[[Category: Cheng, E H.]]
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[[Category: Bird GH]]
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[[Category: Davis, M L.]]
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[[Category: Cheng EH]]
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[[Category: Gavathiotis, E.]]
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[[Category: Davis ML]]
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[[Category: Katz, S G.]]
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[[Category: Gavathiotis E]]
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[[Category: Kim, H.]]
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[[Category: Katz SG]]
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[[Category: Pitter, K.]]
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[[Category: Kim H]]
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[[Category: Suzuki, M.]]
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[[Category: Pitter K]]
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[[Category: Tjandra, N.]]
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[[Category: Suzuki M]]
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[[Category: Tu, H C.]]
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[[Category: Tjandra N]]
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[[Category: Walensky, L D.]]
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[[Category: Tu HC]]
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[[Category: Apoptosis]]
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[[Category: Walensky LD]]
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[[Category: Bax]]
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[[Category: Bcl-2]]
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[[Category: Bh3]]
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[[Category: Bim]]
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Current revision

BAX Activation is Initiated at a Novel Interaction Site

PDB ID 2k7w

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