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2jz4

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[[Image:2jz4.png|left|200px]]
 
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{{STRUCTURE_2jz4| PDB=2jz4 | SCENE= }}
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==Putative 32 kDa myrosinase binding protein At3g16450.1 from Arabidopsis thaliana==
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<StructureSection load='2jz4' size='340' side='right'caption='[[2jz4]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JZ4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jz4 OCA], [https://pdbe.org/2jz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jz4 RCSB], [https://www.ebi.ac.uk/pdbsum/2jz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jz4 ProSAT], [https://www.topsan.org/Proteins/CESG/2jz4 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/JAL33_ARATH JAL33_ARATH] Sugar-binding protein showing significant affinity for (Glc alpha(1-4)Glc)(3) maltohexaose, (Glc alpha(1-6)Glc)(3) isomaltohexaose, Gal alpha(1-4)Gal beta(1-4)Glc, GalNAc alpha(1-3)(Fuc alpha(1-2)) and Gal beta(1-3)(Fuc alpha(1-4))GlcNAc beta(1-3)Gal beta(1-4)Glc.<ref>PMID:19021763</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jz/2jz4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jz4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The product of gene At3g16450.1 from Arabidopsis thaliana is a 32 kDa, 299-residue protein classified as resembling a myrosinase-binding protein (MyroBP). MyroBPs are found in plants as part of a complex with the glucosinolate-degrading enzyme myrosinase, and are suspected to play a role in myrosinase-dependent defense against pathogens. Many MyroBPs and MyroBP-related proteins are composed of repeated homologous sequences with unknown structure. We report here the three-dimensional structure of the At3g16450.1 protein from Arabidopsis, which consists of two tandem repeats. Because the size of the protein is larger than that amenable to high-throughput analysis by uniform (13)C/(15)N labeling methods, we used stereo-array isotope labeling (SAIL) technology to prepare an optimally (2)H/(13)C/(15)N-labeled sample. NMR data sets collected using the SAIL protein enabled us to assign (1)H, (13)C and (15)N chemical shifts to 95.5% of all atoms, even at a low concentration (0.2 mm) of protein product. We collected additional NOESY data and determined the three-dimensional structure using the cyana software package. The structure, the first for a MyroBP family member, revealed that the At3g16450.1 protein consists of two independent but similar lectin-fold domains, each composed of three beta-sheets.
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===Putative 32 kDa myrosinase binding protein At3g16450.1 from Arabidopsis thaliana===
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Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR.,Takeda M, Sugimori N, Torizawa T, Terauchi T, Ono AM, Yagi H, Yamaguchi Y, Kato K, Ikeya T, Jee J, Guntert P, Aceti DJ, Markley JL, Kainosho M FEBS J. 2008 Dec;275(23):5873-84. PMID:19021763<ref>PMID:19021763</ref>
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{{ABSTRACT_PUBMED_19021763}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2jz4" style="background-color:#fffaf0;"></div>
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[[2jz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JZ4 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019021763</ref><references group="xtra"/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Aceti, D J.]]
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[[Category: Large Structures]]
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[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
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[[Category: Aceti DJ]]
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[[Category: Guntert, P.]]
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[[Category: Guntert P]]
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[[Category: Ikeya, T.]]
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[[Category: Ikeya T]]
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[[Category: Kainosho, M.]]
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[[Category: Kainosho M]]
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[[Category: Kato, K.]]
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[[Category: Kato K]]
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[[Category: Markley, J L.]]
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[[Category: Markley JL]]
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[[Category: Ono, A M.]]
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[[Category: Ono AM]]
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[[Category: Sugimori, N.]]
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[[Category: Sugimori N]]
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[[Category: Takeda, N.]]
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[[Category: Takeda N]]
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[[Category: Terauchi, T.]]
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[[Category: Terauchi T]]
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[[Category: Torizawa, T.]]
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[[Category: Torizawa T]]
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[[Category: Yagi, H.]]
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[[Category: Yagi H]]
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[[Category: Yamaguchi, Y.]]
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[[Category: Yamaguchi Y]]
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[[Category: At3g16450 1]]
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[[Category: Center for eukaryotic structural genomic]]
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[[Category: Cesg]]
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[[Category: Myrosinase binding protein]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Sail]]
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[[Category: Stereo-array isotope labeling]]
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[[Category: Structural genomic]]
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[[Category: Unknown function]]
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Current revision

Putative 32 kDa myrosinase binding protein At3g16450.1 from Arabidopsis thaliana

PDB ID 2jz4

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