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1h14

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[[Image:1h14.gif|left|200px]]<br />
 
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<applet load="1h14" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h14, resolution 1.50&Aring;" />
 
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'''STRUCTURE OF A COLD-ADAPTED FAMILY 8 XYLANASE'''<br />
 
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==Overview==
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==Structure of a cold-adapted family 8 xylanase==
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Enzymes from psychrophilic organisms differ from their mesophilic, counterparts in having a lower thermostability and a higher specific, activity at low and moderate temperatures. The current consensus is that, they have an increased flexibility, enhancing accommodation and, transformation of the substrates at low energy costs. Here we describe the, structure of the xylanase from the Antarctic bacterium Pseudoalteromonas, haloplanktis at 1.3 A resolution. Xylanases are usually grouped into, glycosyl hydrolase families 10 and 11, but this enzyme belongs to family, 8. The fold differs from that of other known xylanases and can be, described as an (alpha/alpha)(6) barrel. Various parameters that may, explain the cold-adapted properties were examined and indicated that the, protein has a ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12475991 (full description)]]
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<StructureSection load='1h14' size='340' side='right'caption='[[1h14]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h14]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_haloplanktis Pseudoalteromonas haloplanktis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H14 FirstGlance]. <br>
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1H14 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudoalteromonas_haloplanktis Pseudoalteromonas haloplanktis]]. Active as [[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H14 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h14 OCA], [https://pdbe.org/1h14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h14 RCSB], [https://www.ebi.ac.uk/pdbsum/1h14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h14 ProSAT]</span></td></tr>
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==Reference==
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</table>
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The structure of a cold-adapted family 8 xylanase at 1.3 A resolution. Structural adaptations to cold and investgation of the active site., Van Petegem F, Collins T, Meuwis MA, Gerday C, Feller G, Van Beeumen J, J Biol Chem. 2003 Feb 28;278(9):7531-9. Epub 2002 Dec 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12475991 12475991]
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== Function ==
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[[Category: Endo-1,4-beta-xylanase]]
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[https://www.uniprot.org/uniprot/Q8RJN8_PSEHA Q8RJN8_PSEHA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h1/1h14_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h14 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudoalteromonas haloplanktis]]
[[Category: Pseudoalteromonas haloplanktis]]
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[[Category: Single protein]]
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[[Category: Collins T]]
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[[Category: Beeumen, J.Van.]]
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[[Category: Feller G]]
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[[Category: Collins, T.]]
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[[Category: Gerday C]]
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[[Category: Feller, G.]]
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[[Category: Meuwis MA]]
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[[Category: Gerday, C.]]
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[[Category: Van Beeumen J]]
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[[Category: Meuwis, M.A.]]
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[[Category: Van Petegem F]]
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[[Category: Petegem, F.Van.]]
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[[Category: cold adaptation]]
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[[Category: family 8]]
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[[Category: glycosyl hydrolase]]
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[[Category: hydrolase]]
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[[Category: psychrophilic]]
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[[Category: temperature]]
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[[Category: xylan degradation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:25:14 2007''
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Current revision

Structure of a cold-adapted family 8 xylanase

PDB ID 1h14

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