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1ww1

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[[Image:1ww1.gif|left|200px]]<br /><applet load="1ww1" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ww1, resolution 2.60&Aring;" />
 
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'''Crystal structure of tRNase Z from Thermotoga maritima'''<br />
 
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==Overview==
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==Crystal structure of tRNase Z from Thermotoga maritima==
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The maturation of the tRNA 3' end is catalyzed by a tRNA 3' processing endoribonuclease named tRNase Z (RNase Z or 3'-tRNase) in eukaryotes, Archaea, and some bacteria. The tRNase Z generally cuts the 3' extra sequence from the precursor tRNA after the discriminator nucleotide. In contrast, Thermotoga maritima tRNase Z cleaves the precursor tRNA precisely after the CCA sequence. In this study, we determined the crystal structure of T. maritima tRNase Z at 2.6-A resolution. The tRNase Z has a four-layer alphabeta/betaalpha sandwich fold, which is classified as a metallo-beta-lactamase fold, and forms a dimer. The active site is located at one edge of the beta-sandwich and is composed of conserved motifs. Based on the structure, we constructed a docking model with the tRNAs that suggests how tRNase Z may recognize the substrate tRNAs.
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<StructureSection load='1ww1' size='340' side='right'caption='[[1ww1]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ww1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WW1 FirstGlance]. <br>
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1WW1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_Z Ribonuclease Z], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.11 3.1.26.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WW1 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ww1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ww1 OCA], [https://pdbe.org/1ww1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ww1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ww1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ww1 ProSAT], [https://www.topsan.org/Proteins/RSGI/1ww1 TOPSAN]</span></td></tr>
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Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima., Ishii R, Minagawa A, Takaku H, Takagi M, Nashimoto M, Yokoyama S, J Biol Chem. 2005 Apr 8;280(14):14138-44. Epub 2005 Jan 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15701599 15701599]
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</table>
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[[Category: Ribonuclease Z]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q9WZW8_THEMA Q9WZW8_THEMA]
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[[Category: Thermotoga maritima]]
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== Evolutionary Conservation ==
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[[Category: Ishii, R.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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Check<jmol>
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[[Category: Yokoyama, S.]]
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<jmolCheckbox>
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[[Category: ZN]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ww/1ww1_consurf.spt"</scriptWhenChecked>
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[[Category: metallo-beta-lactamase fold]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: riken structural genomics/proteomics initiative]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: rsgi]]
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</jmolCheckbox>
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[[Category: structural genomics]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ww1 ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:48:39 2008''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermotoga maritima MSB8]]
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[[Category: Ishii R]]
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[[Category: Yokoyama S]]

Current revision

Crystal structure of tRNase Z from Thermotoga maritima

PDB ID 1ww1

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