2fmd

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[[Image:2fmd.png|left|200px]]
 
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{{STRUCTURE_2fmd| PDB=2fmd | SCENE= }}
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==Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin==
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<StructureSection load='2fmd' size='340' side='right'caption='[[2fmd]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fmd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leucomphalos_mildbraedii Leucomphalos mildbraedii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FMD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASX:ASP/ASN+AMBIGUOUS'>ASX</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900111:2alpha-alpha-mannobiose'>PRD_900111</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fmd OCA], [https://pdbe.org/2fmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fmd RCSB], [https://www.ebi.ac.uk/pdbsum/2fmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fmd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEC_LEUMI LEC_LEUMI] Binds preferentially to oligosaccharides bearing the sequence Man-alpha-1->2 Man-alpha-1->6 Man-alpha-1->6Man found in early steps of glycoprotein processing in the endoplasmic reticulum. It binds weakly to highly processed oligosaccharide structures.<ref>PMID:2925660</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/2fmd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fmd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the seed lectin from the tropical legume Bowringia milbraedii was determined in complex with the disaccharide ligand Man(alpha1-2)Man. In solution, the protein exhibits a dynamic dimer-tetramer equilibrium, consistent with the concanavalin A-type tetramer observed in the crystal. Contacts between the tetramers are mediated almost exclusively through the carbohydrate ligand, resulting in a crystal lattice virtually identical to that of the concanavalin-A:Man(alpha1-2)Man complex, even though both proteins have less than 50% sequence identity. The disaccharide binds exclusively in a "downstream" binding mode, with the non-reducing mannose occupying the monosaccharide-binding site. The reducing mannose is bound in a predominantly polar subsite involving Tyr131, Gln218, and Tyr219.
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===Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin===
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Structural basis of carbohydrate recognition by a Man(alpha1-2)Man-specific lectin from Bowringia milbraedii.,Buts L, Garcia-Pino A, Wyns L, Loris R Glycobiology. 2006 Jul;16(7):635-40. Epub 2006 Mar 27. PMID:16567368<ref>PMID:16567368</ref>
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{{ABSTRACT_PUBMED_16567368}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2fmd" style="background-color:#fffaf0;"></div>
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[[2fmd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leucomphalos_mildbraedii Leucomphalos mildbraedii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMD OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:016567368</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Leucomphalos mildbraedii]]
[[Category: Leucomphalos mildbraedii]]
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[[Category: Buts, L.]]
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[[Category: Buts L]]
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[[Category: Garcia-Pino, A.]]
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[[Category: Garcia-Pino A]]
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[[Category: Loris, R.]]
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[[Category: Loris R]]
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[[Category: Wyns, L.]]
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[[Category: Wyns L]]
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[[Category: Beta sandwich]]
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[[Category: Lectin]]
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[[Category: Legume lectin]]
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[[Category: Protein-carbohydrate complex]]
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[[Category: Sugar binding protein]]
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Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin

PDB ID 2fmd

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