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2o0f

From Proteopedia

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[[Image:2o0f.png|left|200px]]
 
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{{STRUCTURE_2o0f| PDB=2o0f | SCENE= }}
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==Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3==
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<SX load='2o0f' size='340' side='right' viewer='molstar' caption='[[2o0f]], [[Resolution|resolution]] 15.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2o0f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O0F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 15.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o0f OCA], [https://pdbe.org/2o0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o0f RCSB], [https://www.ebi.ac.uk/pdbsum/2o0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o0f ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RF3_ECOLI RF3_ECOLI] Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.[HAMAP-Rule:MF_00072]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o0/2o0f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o0f ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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During translation termination, class II release factor RF3 binds to the ribosome to promote rapid dissociation of a class I release factor (RF) in a GTP-dependent manner. We present the crystal structure of E. coli RF3*GDP, which has a three-domain architecture strikingly similar to the structure of EF-Tu*GTP. Biochemical data on RF3 mutants show that a surface region involving domains II and III is important for distinct steps in the action cycle of RF3. Furthermore, we present a cryo-electron microscopy (cryo-EM) structure of the posttermination ribosome bound with RF3 in the GTP form. Our data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center of the ribosome, apparently leading to the release of the class I RF.
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===Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3===
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RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors.,Gao H, Zhou Z, Rawat U, Huang C, Bouakaz L, Wang C, Cheng Z, Liu Y, Zavialov A, Gursky R, Sanyal S, Ehrenberg M, Frank J, Song H Cell. 2007 Jun 1;129(5):929-41. PMID:17540173<ref>PMID:17540173</ref>
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{{ABSTRACT_PUBMED_17540173}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2o0f" style="background-color:#fffaf0;"></div>
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[[2o0f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O0F OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:017540173</ref><references group="xtra"/>
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</SX>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Bouakaz, L.]]
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[[Category: Large Structures]]
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[[Category: Ehrenberg, M.]]
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[[Category: Bouakaz L]]
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[[Category: Frank, J.]]
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[[Category: Ehrenberg M]]
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[[Category: Gao, H.]]
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[[Category: Frank J]]
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[[Category: Gursky, R.]]
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[[Category: Gao H]]
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[[Category: Huang, C.]]
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[[Category: Gursky R]]
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[[Category: Liu, Y.]]
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[[Category: Huang C]]
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[[Category: Rawat, U.]]
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[[Category: Liu Y]]
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[[Category: Sanyal, S.]]
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[[Category: Rawat U]]
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[[Category: Song, H.]]
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[[Category: Sanyal S]]
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[[Category: Wang, C.]]
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[[Category: Song H]]
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[[Category: Zavialov, A.]]
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[[Category: Wang C]]
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[[Category: Zhou, Z.]]
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[[Category: Zavialov A]]
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[[Category: Cryo-em]]
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[[Category: Zhou Z]]
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[[Category: Rf3]]
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[[Category: Ribosome]]
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[[Category: Translation]]
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Current revision

Docking of the modified RF3 X-ray structure into cryo-EM map of E.coli 70S ribosome bound with RF3

2o0f, resolution 15.50Å

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OCA

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