2jwn

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[[Image:2jwn.png|left|200px]]
 
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{{STRUCTURE_2jwn| PDB=2jwn | SCENE= }}
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==Solution NMR structure of the protease-resistent domain of Xenopus laevis ePABP2==
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<StructureSection load='2jwn' size='340' side='right'caption='[[2jwn]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jwn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JWN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jwn OCA], [https://pdbe.org/2jwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jwn RCSB], [https://www.ebi.ac.uk/pdbsum/2jwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jwn ProSAT], [https://www.topsan.org/Proteins/CESG/2jwn TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EPA2B_XENLA EPA2B_XENLA] Binds the poly(A) tail of mRNA. Unable to interact with the cap-binding complex and is therefore unlikely to be involved in translation initiation.<ref>PMID:15083517</ref> [UniProtKB:Q804A5]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/2jwn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jwn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We identified a functional domain (XlePABP2-TRP) of Xenopus laevis embryonic type II poly(A)-binding protein (XlePABP2). The NMR structure of XlePABP2-TRP revealed that the protein is a homodimer formed by the antiparallel association of beta-strands from the single RNA recognition motif (RRM) domain of each subunit. In each subunit of the homodimer, the canonical RNA recognition site is occluded by a polyproline motif. Upon poly(A) binding, XlePABP2-TRP undergoes a dimer-monomer transition that removes the polyproline motif from the RNA recognition site and allows it to be replaced by the adenosine nucleotides of poly(A). Our results provide high-resolution structural information concerning type II PABPs and an example of a single RRM domain protein that transitions from a homodimer to a monomer upon RNA binding. These findings advance our understanding of RRM domain regulation, poly(A) recognition, and are relevant to understanding how type II PABPs function in mRNA processing and human disease.
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===Solution NMR structure of the protease-resistent domain of Xenopus laevis ePABP2===
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Structural basis for RNA recognition by a type II poly(A)-binding protein.,Song J, McGivern JV, Nichols KW, Markley JL, Sheets MD Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15317-22. Epub 2008 Sep 29. PMID:18824697<ref>PMID:18824697</ref>
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{{ABSTRACT_PUBMED_18824697}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2jwn" style="background-color:#fffaf0;"></div>
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[[2jwn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JWN OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:018824697</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
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[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
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[[Category: Markley JL]]
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[[Category: Markley, J L.]]
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[[Category: Song J]]
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[[Category: Song, J.]]
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[[Category: Center for eukaryotic structural genomic]]
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[[Category: Cesg]]
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[[Category: Cytoplasm rna-binding]]
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[[Category: Epabp2]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Rna binding protein]]
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[[Category: Structural genomic]]
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Current revision

Solution NMR structure of the protease-resistent domain of Xenopus laevis ePABP2

PDB ID 2jwn

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