2kb3

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[[Image:2kb3.png|left|200px]]
 
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{{STRUCTURE_2kb3| PDB=2kb3 | SCENE= }}
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==NMR Structure of the phosphorylated form of OdhI, pOdhI.==
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<StructureSection load='2kb3' size='340' side='right'caption='[[2kb3]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2kb3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KB3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 30 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb3 OCA], [https://pdbe.org/2kb3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kb3 RCSB], [https://www.ebi.ac.uk/pdbsum/2kb3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kb3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ODHI_CORGL ODHI_CORGL] An essential component of the PknG signaling pathway. When unphosphorylated, it inhibits the activity of 2-oxoglutarate dehydrogenase. When phosphorylated it does not inhibit 2-oxoglutarate dehydrogenase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kb3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kb3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The OdhI protein is key regulator of the TCA cycle in Corynebacterium glutamicum. This highly conserved protein is found in GC rich Gram-positive bacteria (e.g., the pathogenic Mycobacterium tuberculosis). The unphosphorylated form of OdhI inhibits the OdhA protein, a key enzyme of the TCA cycle, whereas the phosphorylated form is inactive. OdhI is predicted to be mainly a single FHA domain, a module that mediates protein-protein interaction through binding of phosphothreonine peptides, with a disordered N-terminal extension substrate of the serine/threonine protein kinases. In this study, we solved the solution structure of the unphosphorylated and phosphorylated isoforms of the protein. We observed a major conformational change between the two forms characterized by the binding of the phosphorylated N-terminal part of the protein to its own FHA domain, consequently inhibiting it. This structural observation corresponds to a new autoinhibition mechanism described for a FHA domain protein.
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===NMR Structure of the phosphorylated form of OdhI, pOdhI.===
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Dynamic and structural characterization of a bacterial FHA protein reveals a new autoinhibition mechanism.,Barthe P, Roumestand C, Canova MJ, Kremer L, Hurard C, Molle V, Cohen-Gonsaud M Structure. 2009 Apr 15;17(4):568-78. PMID:19368890<ref>PMID:19368890</ref>
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{{ABSTRACT_PUBMED_19368890}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2kb3" style="background-color:#fffaf0;"></div>
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[[2kb3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB3 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019368890</ref><references group="xtra"/>
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</StructureSection>
[[Category: Corynebacterium glutamicum]]
[[Category: Corynebacterium glutamicum]]
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[[Category: Barthe, P.]]
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[[Category: Large Structures]]
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[[Category: Canova, M.]]
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[[Category: Barthe P]]
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[[Category: Cohen-Gonsaud, M.]]
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[[Category: Canova M]]
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[[Category: Hurard, C.]]
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[[Category: Cohen-Gonsaud M]]
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[[Category: Molle, V.]]
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[[Category: Hurard C]]
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[[Category: Roumestand, C.]]
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[[Category: Molle V]]
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[[Category: Dehydrogenase inhibitor]]
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[[Category: Roumestand C]]
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[[Category: Fha]]
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[[Category: Forkhead-associated domain]]
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[[Category: Gara]]
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[[Category: Kinase substrate]]
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[[Category: Phosphoprotein]]
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Current revision

NMR Structure of the phosphorylated form of OdhI, pOdhI.

PDB ID 2kb3

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